Urine Sediment Stain Characteristics

Question 1

Delineates structure and contrasting
colors of the nucleus and cytoplasm

Answer 1

Sternheimer-Malbin

Question 2

function: Identifies WBCs, epithelial cells, and casts

Answer 2

Sternheimer-Malbin

Question 3

Enhances nuclear detail

Answer 3

Toluidine blue

Question 4

function: Differentiates WBCs from RTE cells

Answer 4

Toluidine blue

Question 5

Lyses RBCs and enhances nuclei of
WBCs

Answer 5

2% acetic acid

Question 6

function: Distinguishes RBCs from WBCs, yeast, oil droplets, and crystals

Answer 6

2% acetic acid

Question 7

Stain triglycerides and neutral fats
orange-red; do not stain cholesterol

Answer 7

Oil Red O and Sudan III

Question 8

function: Identify free fat droplets and lipid-containing cells and casts

Answer 8

Oil Red O and Sudan III

Question 9

Differentiates gram-positive and gram
negative bacteria

Answer 9

Gram stain

Question 10

function: Identifies bacterial casts

Answer 10

Gram stain

Question 11

Methylene blue and eosin Y stains
eosinophilic granules

Answer 11

Hansel stain

Question 12

function: Identifies urinary eosinophils

Answer 12

Hansel stain

Question 13

Stains structures containing iron

Answer 13

Prussian blue stain

Question 14

function: Identifies yellow-brown granules of hemosiderin in cells and casts

Answer 14

Prussian blue stain