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[CC1 LAB] > UREA AND CREATININE > Flashcards

UREA AND CREATININE Flashcards

1
Q

= measures Blood Urea Nitrogen

A

INDIRECT METHOD / ENZYMATIC

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2
Q

Based on the preliminary hydrolysis of urea with urease followed by some process that quantitates the ammonium ion

A

INDIRECT METHOD / ENZYMATIC

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3
Q

INDIRECT METHOD / ENZYMATIC OF UREA DETERMINATION

A
  1. Berthelot reaction
  2. Nessler’s Reaction
  3. GLDH-coupled enzymatic method
  4. Conductimetric method
  5. Urograph or Urastrat strip
  6. Indicator dye (uriol)
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4
Q

Dupont ACA Analyzer

A
  1. GLDH-coupled enzymatic method
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5
Q

Beckman BUN Analyzer

A
  1. Conductimetric method
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6
Q

Kodak Ectachem Analyzer

A
  1. Indicator dye (uriol)
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7
Q

Decrease in absorbance of NAD at 340 nm

A

GLDH-coupled enzymatic method

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8
Q

Based on the measurement of the conductivity generated from the reaction of urease on urea producing ammonium ions & bicarbonates

A

Conductimetric method

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9
Q

Urograph or Urastrat strip Chemical principle:

A

Conway Microdiffusion method

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10
Q

Urograph or Urastrat strip Physical principle:

A

based on chromatography

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11
Q

Dye is added to NH4 ions from urea hydrolysis & the color change is measured

A

Indicator dye (uriol)

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12
Q

Used in multilayer film reagents, dry reagent strips and automated systems

A

Indicator dye (uriol)

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13
Q

DIRECT METHODS OF UREA DETERMINATION

A
  1. Diacetyl Monoxime
  2. ortho –phthaldehyde
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14
Q

Fearon

A

Diacetyl Monoxime

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15
Q

Direct condensation reaction

A

Diacetyl Monoxime

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16
Q

very toxic

A

Diacetyl

17
Q

: adapted by automated methods

A

ortho – phthaldehyde

18
Q

Urea + OP —>

A

Isoindoline

19
Q

Isoindoline + [?] —-> colored compound

A

Naphthylethylenediamine

20
Q

DIRECT METHOD OF CREATININE DETERMINATION

A

JAFFE REACTION

21
Q

INDIRECT / ENZYMATIC METHODS OF CREATININE DETERMINATION

A

F. Lim – Creatininase or creatinine iminohydrolase
G.A. Moss – Creatinine Amidohydrolase

22
Q

Creatinine reacts with alkaline picrate at two different pH levels

A

Yatzidis method

23
Q

: protein & other interfering materials will reacts w/ picrate but creatinine does not

A

pH 10

24
Q

: both creatinine & proteins react

A

pH 11

25
Q

Sources of error :

A
  1. ascorbate, glucose, alpha keto acids and uric acid – false increase
  2. drugs
    : cephalosporin and dopamine intake – false increase
    : lidocaine intake – false increase
26
Q

Creatinine + alkaline picrate —–>

A

Creatinine picrate (red orange/yellow) 510 nm

27
Q

Alkaline picrate: 1 part [?] and 5 parts [?] (2,4,6 trinitrophenol)

A

10% NaOH
sat. picric acid

28
Q

lacks specificity

A

Jaffe reaction

29
Q

Non-creatinine Jaffe-reacting chromogens:

A
  • Proteins
  • Glucose
  • Ascorbic acid
  • Guanidine
  • Acetone
  • Cephalosporins
  • α-ketoacids (acetoacetate and pyruvate)

[CC1 LAB] (5 decks)

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