Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

CC1 LAB > METHODS > Flashcards

METHODS Flashcards

1
Q

glucose + boiling water —-> eneidol (enol anion form)

A

Chemical methods : oxidation-reduction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

phosphomolybdic acid —> phosphomolybdenum blue

A

Folin-Wu

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

arsenomolybdic acid —> arsenomolybdenum blue

A

Nelson-Somogyi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

neocuproine (7,9- dimethyl-1,10-phenanthroline) —> cuprous-neocuproine complex (yellow-orange)

A

Campbell and King method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

ferricyanide —> ferrocyanide

A

reverse colorimetric method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

glucose + ortho-toluidine —-> N-glycosylamine: Schiff base (green) 630 nm

A

Ortho-toluidine method by Dubowski

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

glucose + O2 + H2O — GOD —> gluconic acid + H2O2

A

Glucose Oxidase method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

H2O2 + reduced chromogen — POD—> oxidized chromogen + H2O

A

Glucose Oxidase method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

3-methyl-2-benzathiazolinone hydrazone + N,N-dimethylaniline – -> indamine dye (590-66- nm)

A

Gochman and Schmitz

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

p-aminophenazone + phenol —-> purple product (quinoneimine)

A

Trinder

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

2,2-azine-di(3-ethyl bezothiazoline-(6)-sulfonic acid —> colored chromogen

A

Miskieweis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

glucose + ATP —hexokinase —> glu-6-phosphate + ADP

A

Hexokinase method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

glu-6-phosphate + NADP —G-6-PD —> NADPH + H + 6-phosphogluconate (340 nm)

A

Hexokinase method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Glucose + NAD —— GDH —– Gluconolactone + NADH + H+

A

Glucose Dehydrogenase (GDH)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Hb A1c — acid — 5-HMF

A

COLORIMETRY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

acetoacetic acid + ferric chloride —> red color

A

Gerhardt

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

acetoacetic acid + NADH + H —β-HBDH—> NAD + beta-hydroxybutyric acid

A

enzymatic method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

NPN + H2SO4 NH4HSO4

A

KJELDAHL DIGESTION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

NH3 + HgI2.2KI NH2Hg2I2 + KI + NH4I

A

NESSLERIZATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

NH3 + NaOCl + Phenol Indophenol + NaCl + H2O

A

BERTHELOT METHOD

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

NH3 + α-ketoglutarate + NADH + H Glutamate + NAD

A

Kaplan, Manoukian – Fawaz; Kallet – Cook Reaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Isoindoline + Naphthylethylenediamine colored compound

A

Diacetyl Monoxime (Fearon)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Creatinine + alkaline picrate Creatinine picrate (red orange/yellow)

A

JAFFE REACTION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Creatinine —————-→N-methylhydantoin + NH3

A

F. Lim

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Creatinine -----------------------------------→Creatine (Creatinine amidohydrolase)
G.A. Moss
26
Uric Acid + PTA ------- OH-------- Allantoin + CO2 + Tungsten blue (710 nm)
Phosphotungstic Acid (PTA)
27
Uric Acid Allantoin + CO2
Blaunch and Koch
28
Uric Acid + O2 + 2H2O Allantoin + CO2 + H2O2
Trinder –Uricase method
29
Uric Acid Allantoin + CO2 + H2O2
Uricase –catalase system
30
Cuprous ions + neocuproine copper neocuproine complex
Bittner method
31
Ferrous ions + TPTZ blue colored complex (590 nm)
TPTZ Method by Morin
32
specimen is alkalinized to convert NH4 ions to NH3
Conway and Cook Diffusion Method
33
NH3 is trapped in acid medium of diffusion cell
Conway and Cook Diffusion Method
34
Quantitated by titration or colorimetry
Conway and Cook Diffusion Method
35
Time consuming with poor accuracy and precision
Conway and Cook Diffusion Method
36
Uses cation-exchange resin
Forman’s Resin Absorption Method
37
NH3 absorbed by the resin and eluted
Forman’s Resin Absorption Method
38
Quantitated by Berthelot reaction or by Nesslerization
Forman’s Resin Absorption Method
39
Forman’s Resin Absorption Method N.V.:
16 - 33 µmol/L
40
NH3 is obtained through the use of a Dowax column
Kunahashi, Ishihora and Euhera Method
41
Assayed using the Berthelot method
Kunahashi, Ishihora and Euhera Method
42
2-oxoglutarate + NH4+ + NADPH Glutamate + NADP + H2O
Van Anken Enzymatic Method
43
Van Anken Enzymatic Method N.V.:
11 –35 µmol/L
44
Based on the diffusion of NH3 through a selective membrane into NH4 chloride causing pH change which is determined potentiometrically
Ion Selective Electrode
45
Good precision and accuracy
Ion Selective Electrode
46
3 FA + glycerol
Complete Hydrolysis
47
Partial Hydrolysis
2 FA + monoglyceride
48
serum is combined with the color reagent then incubated to allow color reaction to occur
Direct Colorimetric / One-Step
49
Methods: Watson’s, Ferro-Ham, Pearson, Zlatkis
Direct Colorimetric / One-Step
50
Reagents: Acid anhydride, conc. H2SO4
Liebermann-Burchard Reaction
51
End-product: cholestapolyene sulfonic acid (emerald green)
Liebermann-Burchard Reaction
52
Reagents: conc. HAc, Ferric ions
Salkowski’s Reaction
53
End-product: cholestapolyene carbonium ion (reddish-purple)
Salkowski’s Reaction
54
Involves 1extraction step prior to 2colorimetric reaction
Two-Step Method
55
Eliminates protein interference
Two-Step Method
56
Methods: Carr-Drekter, Saifer-Kammer, Leoffler-McDougold
Two-Step Method
57
Requires 1extraction w/ petroleum ether followed by 2 saponification and then 3colorimetric determination
Three-Step Method
58
Standard method
Three-Step Method
59
Method: Abell-Kendall
Three-Step Method
60
Requires 1extraction, 2saponification, 3purification with digitonin then 4colorimetric determination
Four-Step Method
61
Considered as the reference method
Four-Step Method
62
Methods: Schoenheimer-Sperry, Sperry-Webb
Four-Step Method
63
cholesteryl ester + water ----cholesterol esterase ----> cholesterol + FFA
ENZYMATIC METHODS
64
cholesterol + O2 ----cholesterol oxidase----> cholest-4-en-3-one + H2O2
ENZYMATIC METHODS
65
Formaldehyde + acetylacetone + NH4 3,5-diacetyl-1,4-dihydrolutidine
Hantzsch-Lutidine Reaction
66
Formaldehyde + H2SO4 + chromotropic acid (CTA) pink chromophore
Van Handel and Zilversmit
67
NADH + tetrazolium dye ---diaphorase---> formazan + NAD
Weiland Method
68
glycerophosphate + O2 ---glycerophosphate oxidase---> DHAP + H2O2
Trinder Reaction
69
H202 + reduced chromogen --→Quinoneimine dye
Trinder Reaction
70
ADP + PEP ---pyruvate kinase ---> ATP + pyruvate
Eggstein and Kreutz method
71
pyruvate + NADH + H ---LDH---> lactate + NAD (340 nm)
Eggstein and Kreutz method
72
intermittent hyperbilirubinemia;
Gilbert’s Syndrome
73
cause by reduction in the expression of UGT1A1 gene
Gilbert’s Syndrome
74
Chronic unconjugated hyperbilirubinemia; rare & more severe disorder
Crigler-Najjar Syndrome
75
Molecular defect w/in the gene involved with bilirubin conjugation
Crigler-Najjar Syndrome
76
complete absence of enzymatic bilirubin conjugation
Type I
77
mutation causing a severe deficiency of the enzyme for conjugation
Type II
78
Removal of conjugated bilirubin from the liver cell & the excretion into the bile are defective
Dubin-Johnson Syndrome
79
Caused by deficiency of the canalicular transporter protein (MDR2/cMOAT)
Dubin-Johnson Syndrome
80
Appearance of dark-stained granules on a liver biopsy
Dubin-Johnson Syndrome
81
Hypothesized to be due to a reduction in the concentration or activity of intracellular binding proteins
Rotor Syndrome
82
Liver biopsy does NOT show dark pigmented granules
Rotor Syndrome
83
Cirrhosis
Beta-gamma bridging effect
84
Monoclonal gammopathy (Multiple myeloma)
Monoclonal band
85
Chronic inflammation
Polyclonal band
86
nephrotic syndrome
Increase in α-2-macroglobulin
87
deficiency in α-1-antitrypsin
a-1-globulin flat curve
88
Juvenile cirrhosis
a-1-globulin flat curve

CC1 LAB (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions