Unusual Bacteria

Question 1

Mycoplasma

Answer 1

1. No cell wall
2. Cholesterol in cell mem
3. Smallest self-replicating organism
4. Small genome
5. Parasitic life-style in resp and UG tracts
6. Walking pneumonia
7. Immunity short lived

Question 2

Mycobacterium

Answer 2

1. Mycolic acid = reason for hardiness
2. Acid fast stain
3. Slow growing (incubate at least 5 days)
4. Gram (+) bacilli
5. Immobile, aerobic
6. M. Tuberculosis: Lowenstein-Jenson medium
7. M. Leprae
8. M. Avium complex

Question 3

Acid-fast stain

Answer 3

1. Carbol fuchsin
2. 3% HCL
3. Methylene blue

Question 4

Chlamydia

Answer 4

1. Biphasic dev. Cycle
  A. RB = noninfective particle
   1. Stains blue w/ geisma
  B. EB = infective
    1. Stains purple w/ geisma
2. Replication: EB -> RB -> EB
3. UG infections and pneumonia

Question 5

Rickettsia

Answer 5

1. Obligate intracellular parasites
2. Transmitted by arthropods
3. Fever, rash, vasculitis