Unpreserved Urine & Preservation Flashcards

1
Q

In unpreserved urine, color _ due to _ of metabolites.

A

modified/darkened; oxidation or reduction

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2
Q

In unpreserved urine, ph increased due to breakdown of _ to _ by urease-producing bacteria/loss of CO2

A

urea; ammonia

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3
Q

In unpreserved urine, ph increased due to breakdown of urea to ammonia by _ /loss of CO2

A

urease-producing bacteria

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4
Q

In unpreserved urine, odor increases due to _ multiplication causing breakdown of urea to ammonia

A

bacterial

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5
Q

In unpreserved urine, aromatic odor became _

A

ammoniacal

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6
Q

In unpreserved urine, nitrite increases due to multiplication of _

A

nitrate-reducing bacteria

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7
Q

In unpreserved urine, these analytes increase:

A

PONB

  • pH
  • Odor
  • Nitrite
  • Bacteria
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8
Q

T/F. In unpreserved urine, trichomonas decreased due to loss of motility resulting to death.

A

True

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9
Q

In unpreserved urine, trichomonas looks like _

A

WBC

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10
Q

In unpreserved urine, clarity decreased due to _ growth and _ of _ material

A

bacterial; precipitation; amorphous

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11
Q

In unpreserved urine, these analytes decrease:

A
“cge, rub kita”
CGRUBKT
- Clarity
- Glucose
- RBC, WBC, Casts
- Uribilinogen
- Bilirubin
- Ketones
- Trichomonas
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12
Q

In unpreserved urine, glucose decreased due to _ and _ use.

A

glycolysis; bacterial

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13
Q

In unpreserved urine, ketones decreased due to _ and bacterial _

A

volatilization; metabolism

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14
Q

In unpreserved urine, bilirubin decreased due to exposure to / to biliverdin

A

light/photo oxidation

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15
Q

when exposed to light, bilirubin became _

A

biliverdin

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16
Q

In unpreserved urine, urobilinogen decreased due to _ to _

A

oxidation; urobilin

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17
Q

In unpreserved urine, RBC, WBC, Casts decreased due to _ in dilute _ urine

A

disintegration; alkaline

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18
Q

The most routinely used method of preservation is _.

A

refrigeration

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19
Q

Refrigeration temperature:

A

2°C to 8°C

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20
Q

Refrigeration decreases bacterial _ & _.

A

growth & metabolism

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21
Q

The ideal preservative should be _, inhibit _, and preserve formed elements in the sediment.

A

bactericidal; urease

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22
Q

Preservative that does not interfere with chemical tests.

A

Refrigeration

23
Q

Refrigeration can maintain acid pH up about _ hours.

A

8

24
Q

Preservative that precipitates amorphous phosphates (alkaline urine) and urates (acidic urine)

A

Refrigeration

25
Q

Refrigeration prevents bacterial growth for _ hours

A

24

26
Q

Preservative that prevents bacterial growth and metabolism

A

Boric Acid

27
Q

Boric acid interferes with _ and _ analyses

A

drug; hormone

28
Q

Boric acid keeps pH at about _

A

6.0

29
Q

Preservative that can be used as urine culture transport.

A

Boric acid

30
Q

Excellent sediment preservative

A

Formalin (formaldehyde)

31
Q

Boric acid is used for _ section

A

microbiology

32
Q

Acts as a reducing agent, interfering with chemical tests for glucose, blood, leukocyte esterase, and copper reduction

A

Formalin (formaldehyde)

33
Q

Formalin (formaldehyde) interferes with chemical tests for:

A
  • glucose
  • blood
  • leukocyte esterase
  • copper reduction
34
Q

Rinse specimen container with formalin to preserve cells and _

A

casts

35
Q

Is a good preservative for drug analyses

A

Sodium fluoride

36
Q

Sodium fluoride inhibits _ tests

A

reagent strip

37
Q

Sodium fluoride inhibits reagent strip tests for

A
  • glucose
  • blood
  • leukocytes
38
Q

Convenient when refrigeration not possible

A

Commercial preservative tablets

39
Q

Have controlled concentration to minimize interference

A

Commercial preservative tablets

40
Q

Urine Collection Kits

A

Becton, Dickinson, Rutherford, NJ

41
Q

Urine collection kits contains:

A

collection cup, transfer straw, culture and sensitivity (C&S) preservative tube, or UA tube

42
Q

Sample stable at room tempera ture (RT) for 48 hours; prevents bacterial growth and metabolism

A

Light gray and gray C&S tube

43
Q

Light gray and gray C&S tube preservative is

A

boric acid, sodium borate, and sodium formate

44
Q

Keeps pH at about 6.0

A

boric acid; Light gray and gray C&S tube

45
Q

Light gray and gray C&S tubes stable at RT for _ hours

A

48

46
Q

Stable for 72 hours at RT; in- strument-compatible

A

Cherry red/yellow Preservative Plus tube

47
Q

in- strument-compatible

A

Cherry red/yellow Preservative Plus tube

48
Q

Cherry red/yellow Preservative Plus tube is stable at RT for _ hours

A

72

49
Q

Bilirubin and urobilinogen may be decreased if specimen is exposed to light and left at RT

A

Cherry red/yellow Preservative Plus tube

50
Q

Cherry red/yellow Preservative Plus tube preservative is:

A

sodium propionate, ethyl paraben, and chlorhexidine;

51
Q

round or conical bottoms

A

Cherry red/yellow Preservative Plus tube & Yellow UA Plus tube

52
Q

Use on automated instruments

A

Yellow UA Plus tube

53
Q

Yellow UA Plus tube must refrigerated within _ hours

A

2

54
Q

T/F. Yellow UA Plus tube has preservative.

A

False. F. Yellow UA Plus tube has no preservative.