Unpreserved Urine & Preservation Flashcards
In unpreserved urine, color _ due to _ of metabolites.
modified/darkened; oxidation or reduction
In unpreserved urine, ph increased due to breakdown of _ to _ by urease-producing bacteria/loss of CO2
urea; ammonia
In unpreserved urine, ph increased due to breakdown of urea to ammonia by _ /loss of CO2
urease-producing bacteria
In unpreserved urine, odor increases due to _ multiplication causing breakdown of urea to ammonia
bacterial
In unpreserved urine, aromatic odor became _
ammoniacal
In unpreserved urine, nitrite increases due to multiplication of _
nitrate-reducing bacteria
In unpreserved urine, these analytes increase:
PONB
- pH
- Odor
- Nitrite
- Bacteria
T/F. In unpreserved urine, trichomonas decreased due to loss of motility resulting to death.
True
In unpreserved urine, trichomonas looks like _
WBC
In unpreserved urine, clarity decreased due to _ growth and _ of _ material
bacterial; precipitation; amorphous
In unpreserved urine, these analytes decrease:
“cge, rub kita” CGRUBKT - Clarity - Glucose - RBC, WBC, Casts - Uribilinogen - Bilirubin - Ketones - Trichomonas
In unpreserved urine, glucose decreased due to _ and _ use.
glycolysis; bacterial
In unpreserved urine, ketones decreased due to _ and bacterial _
volatilization; metabolism
In unpreserved urine, bilirubin decreased due to exposure to / to biliverdin
light/photo oxidation
when exposed to light, bilirubin became _
biliverdin
In unpreserved urine, urobilinogen decreased due to _ to _
oxidation; urobilin
In unpreserved urine, RBC, WBC, Casts decreased due to _ in dilute _ urine
disintegration; alkaline
The most routinely used method of preservation is _.
refrigeration
Refrigeration temperature:
2°C to 8°C
Refrigeration decreases bacterial _ & _.
growth & metabolism
The ideal preservative should be _, inhibit _, and preserve formed elements in the sediment.
bactericidal; urease
Preservative that does not interfere with chemical tests.
Refrigeration
Refrigeration can maintain acid pH up about _ hours.
8
Preservative that precipitates amorphous phosphates (alkaline urine) and urates (acidic urine)
Refrigeration
Refrigeration prevents bacterial growth for _ hours
24
Preservative that prevents bacterial growth and metabolism
Boric Acid
Boric acid interferes with _ and _ analyses
drug; hormone
Boric acid keeps pH at about _
6.0
Preservative that can be used as urine culture transport.
Boric acid
Excellent sediment preservative
Formalin (formaldehyde)
Boric acid is used for _ section
microbiology
Acts as a reducing agent, interfering with chemical tests for glucose, blood, leukocyte esterase, and copper reduction
Formalin (formaldehyde)
Formalin (formaldehyde) interferes with chemical tests for:
- glucose
- blood
- leukocyte esterase
- copper reduction
Rinse specimen container with formalin to preserve cells and _
casts
Is a good preservative for drug analyses
Sodium fluoride
Sodium fluoride inhibits _ tests
reagent strip
Sodium fluoride inhibits reagent strip tests for
- glucose
- blood
- leukocytes
Convenient when refrigeration not possible
Commercial preservative tablets
Have controlled concentration to minimize interference
Commercial preservative tablets
Urine Collection Kits
Becton, Dickinson, Rutherford, NJ
Urine collection kits contains:
collection cup, transfer straw, culture and sensitivity (C&S) preservative tube, or UA tube
Sample stable at room tempera ture (RT) for 48 hours; prevents bacterial growth and metabolism
Light gray and gray C&S tube
Light gray and gray C&S tube preservative is
boric acid, sodium borate, and sodium formate
Keeps pH at about 6.0
boric acid; Light gray and gray C&S tube
Light gray and gray C&S tubes stable at RT for _ hours
48
Stable for 72 hours at RT; in- strument-compatible
Cherry red/yellow Preservative Plus tube
in- strument-compatible
Cherry red/yellow Preservative Plus tube
Cherry red/yellow Preservative Plus tube is stable at RT for _ hours
72
Bilirubin and urobilinogen may be decreased if specimen is exposed to light and left at RT
Cherry red/yellow Preservative Plus tube
Cherry red/yellow Preservative Plus tube preservative is:
sodium propionate, ethyl paraben, and chlorhexidine;
round or conical bottoms
Cherry red/yellow Preservative Plus tube & Yellow UA Plus tube
Use on automated instruments
Yellow UA Plus tube
Yellow UA Plus tube must refrigerated within _ hours
2
T/F. Yellow UA Plus tube has preservative.
False. F. Yellow UA Plus tube has no preservative.