Unpreserved Urine & Preservation

Question 1

In unpreserved urine, color _ due to _ of metabolites.

Answer 1

modified/darkened; oxidation or reduction

Question 2

In unpreserved urine, ph increased due to breakdown of _ to _ by urease-producing bacteria/loss of CO2

Answer 2

urea; ammonia

Question 3

In unpreserved urine, ph increased due to breakdown of urea to ammonia by _ /loss of CO2

Answer 3

urease-producing bacteria

Question 4

In unpreserved urine, odor increases due to _ multiplication causing breakdown of urea to ammonia

Answer 4

bacterial

Question 5

In unpreserved urine, aromatic odor became _

Answer 5

ammoniacal

Question 6

In unpreserved urine, nitrite increases due to multiplication of _

Answer 6

nitrate-reducing bacteria

Question 7

In unpreserved urine, these analytes increase:

Answer 7

PONB

• pH
• Odor
• Nitrite
• Bacteria

Question 8

T/F. In unpreserved urine, trichomonas decreased due to loss of motility resulting to death.

Answer 8

True

Question 9

In unpreserved urine, trichomonas looks like _

Answer 9

WBC

Question 10

In unpreserved urine, clarity decreased due to _ growth and _ of _ material

Answer 10

bacterial; precipitation; amorphous

Question 11

In unpreserved urine, these analytes decrease:

Answer 11

“cge, rub kita”
CGRUBKT
- Clarity
- Glucose
- RBC, WBC, Casts
- Uribilinogen
- Bilirubin
- Ketones
- Trichomonas

Question 12

In unpreserved urine, glucose decreased due to _ and _ use.

Answer 12

glycolysis; bacterial

Question 13

In unpreserved urine, ketones decreased due to _ and bacterial _

Answer 13

volatilization; metabolism

Question 14

In unpreserved urine, bilirubin decreased due to exposure to / to biliverdin

Answer 14

light/photo oxidation

Question 15

when exposed to light, bilirubin became _

Answer 15

biliverdin

Question 16

In unpreserved urine, urobilinogen decreased due to _ to _

Answer 16

oxidation; urobilin

Question 17

In unpreserved urine, RBC, WBC, Casts decreased due to _ in dilute _ urine

Answer 17

disintegration; alkaline

Question 18

The most routinely used method of preservation is _.

Answer 18

refrigeration

Question 19

Refrigeration temperature:

Answer 19

2°C to 8°C

Question 20

Refrigeration decreases bacterial _ & _.

Answer 20

growth & metabolism

Question 21

The ideal preservative should be _, inhibit _, and preserve formed elements in the sediment.

Answer 21

bactericidal; urease

Question 22

Preservative that does not interfere with chemical tests.

Answer 22

Refrigeration

Question 23

Refrigeration can maintain acid pH up about _ hours.

Answer 23

8

Question 24

Preservative that precipitates amorphous phosphates (alkaline urine) and urates (acidic urine)

Answer 24

Refrigeration

Question 25

Refrigeration prevents bacterial growth for _ hours

Answer 25

24

Question 26

Preservative that prevents bacterial growth and metabolism

Answer 26

Boric Acid

Question 27

Boric acid interferes with _ and _ analyses

Answer 27

drug; hormone

Question 28

Boric acid keeps pH at about _

Answer 28

6.0

Question 29

Preservative that can be used as urine culture transport.

Answer 29

Boric acid

Question 30

Excellent sediment preservative

Answer 30

Formalin (formaldehyde)

Question 31

Boric acid is used for _ section

Answer 31

microbiology

Question 32

Acts as a reducing agent, interfering with chemical tests for glucose, blood, leukocyte esterase, and copper reduction

Answer 32

Formalin (formaldehyde)

Question 33

Formalin (formaldehyde) interferes with chemical tests for:

Answer 33

- glucose - blood - leukocyte esterase - copper reduction

Question 34

Rinse specimen container with formalin to preserve cells and _

Answer 34

casts

Question 35

Is a good preservative for drug analyses

Answer 35

Sodium fluoride

Question 36

Sodium fluoride inhibits _ tests

Answer 36

reagent strip

Question 37

Sodium fluoride inhibits reagent strip tests for

Answer 37

- glucose - blood - leukocytes

Question 38

Convenient when refrigeration not possible

Answer 38

Commercial preservative tablets

Question 39

Have controlled concentration to minimize interference

Answer 39

Commercial preservative tablets

Question 40

Urine Collection Kits

Answer 40

Becton, Dickinson, Rutherford, NJ

Question 41

Urine collection kits contains:

Answer 41

collection cup, transfer straw, culture and sensitivity (C&S) preservative tube, or UA tube

Question 42

Sample stable at room tempera ture (RT) for 48 hours; prevents bacterial growth and metabolism

Answer 42

Light gray and gray C&S tube

Question 43

Light gray and gray C&S tube preservative is

Answer 43

boric acid, sodium borate, and sodium formate

Question 44

Keeps pH at about 6.0

Answer 44

boric acid; Light gray and gray C&S tube

Question 45

Light gray and gray C&S tubes stable at RT for _ hours

Answer 45

48

Question 46

Stable for 72 hours at RT; in- strument-compatible

Answer 46

Cherry red/yellow Preservative Plus tube

Question 47

in- strument-compatible

Answer 47

Cherry red/yellow Preservative Plus tube

Question 48

Cherry red/yellow Preservative Plus tube is stable at RT for _ hours

Answer 48

72

Question 49

Bilirubin and urobilinogen may be decreased if specimen is exposed to light and left at RT

Answer 49

Cherry red/yellow Preservative Plus tube

Question 50

Cherry red/yellow Preservative Plus tube preservative is:

Answer 50

sodium propionate, ethyl paraben, and chlorhexidine;

Question 51

round or conical bottoms

Answer 51

Cherry red/yellow Preservative Plus tube & Yellow UA Plus tube

Question 52

Use on automated instruments

Answer 52

Yellow UA Plus tube

Question 53

Yellow UA Plus tube must refrigerated within _ hours

Answer 53

2

Question 54

T/F. Yellow UA Plus tube has preservative.

Answer 54

False. F. Yellow UA Plus tube has no preservative.