Unit Two Exam: Ch 5 - 10 Flashcards
What is the definition of “sanitization”?
The reduction of the number of microorganisms on a surface or object
If a chemical ends in “-cide,” what kind of chemical is it?
A disinfectant
What type of cage washer is best used for cleaning large numbers of smaller pieces of equipment such as rodent cages, or feed bowls?
A tunnel washer
What is NOT necessarily true about cleaning animal rooms?
A- the animals may need to be removed from the room during cleaning
B- each room should have its own set of cleaning equipment
C- every animal room should be cleaned once per week
D- cleaning and sanitization logs should be filled out after cleaning the room
C- every animal room should be cleaned once per week
Why are vermin a problem in animal rooms?
They can spread disease to the laboratory animals
What must be done before disinfecting an object with bleach?
Debris and organic matter must be removed
What is the most sensitive method to monitor autoclave effectiveness?
Using a biological indicator
What are the three processes used to either remove or kill microorganisms
Sanitization, disinfection, sterilization
What precautions must be taken when autoclaving a liquid substance in a bottle?
Liquid containers should not be tightly sealed or completely filled because the liquid will expand as the temperature rises
Which of the following is an item that should never be autoclaved? A- surgical instrument B- hard plastic mouse cage C- flammable liquid D- cloth gowns
C- flammable liquid
What should you do if you notice that one of the safety valves on the cage washer is not working correctly?
Tell your supervisor immediately
Which Biosafety cabinets are known as “glove boxes”?
Class III
Cleaning assessment using a RODAC (replicate organism detection and counting) plate, involves:
A- microbial growth in a nutrient medium
B- mixing a chemical with swab samples in the room to test for bacteria
C- a magnetic measurement of gravitational pull on surfaces
D- pH strips which indicate the types of microbial growth on surfaces
A- microbial growth in a nutrient medium
Which equipment is often used on loading docks to decontaminate items such as bags of feed and bedding?
Mister
Which equipment can be used to decrease the incidence of ergonomic injuries associated with handling water bottles?
Automated watering system
In an automated watering system, what device mechanically lowers the water pressure for delivery to the individual cages?
Pressure reducing station
What can happen if a drinking valve in a rodent cage remains stuck in the open position?
The cage will flood
How does a laminar flow cabinet protect the worker who is changing the cage?
Air in the cabinet does not exit out the front
A lack of dietary iron can cause what disease?
Anemia
What kind of caging creates a barrier at the cage level, allowing greater control of the individual age environment?
Microisolation
What are squeeze cages used for?
Restraining the animal within its cage
What type of animals are usually housed in a squeeze cage?
Nonhuman primates and hoof stock
Which of the following statements about the size of animal caging is FALSE?
A- an animal must have at least enough room to stand up, lie down, and turn around
B- the size of the floor space includes area occupies by food and water containers
C- the size of the cage is usually based on the animal’s body weight, and other parameters
D- the Guide and the AWR describe minimum cage sizes for most species
B- the size of the floor space includes areas occupied by food and water containers
Which statement is TRUE about individually ventilated cages?
A- bottled water cannot be used with individually ventilated caging systems
B- they cannot be used for biocontainment studies
C- if the ventilation fails, carbon dioxide levels can rise inside the cages and asphyxiate the animals
D- they encourage an exchange of microbes between cages, so extra precautions must be taken
C- if the ventilation fails, carbon dioxide levels can rise inside the cages and asphyxiate the animals
What should be done to rodent shipping containers when they are received?
Spray them down with disinfectant
In general, what type of cage is used to house groups of animals of the same species together?
Group
What is the meaning of the word “microenvironment”?
The environmental conditions within an animal’s cage or pen
What type of animal housing room is usually kept cooler than rodent housing rooms?
Rabbit rooms
Adult mice in rooms that are too warm (>80F) can suffer from:
Heat stress
What is one problem associated with high humidity in an animal room?
A- food can spoil more quickly
B- breeding can be affected
C- bedding may need to be changed less often
D- animals may suffer from dermatitis
A-Food can spoil more quickly
What happens when you open the door to a negative pressure room?
Air rushes into the room from the hall because the pressure is higher in the hallway
What type of room in an animal facility would be kept under positive pressure?
Surgical suite
What is the reason rodents or rabbits are not fasted prior to surgery?
They cannot vomit
How often should watering devices, such as sipper tubes and water valves, be checked to ensure they are in working order?
Daily
What is the worst possible outcome if a rack of animals is disconnected from their water supply?
They could die
How often should soiled bedding be removed and replaced?
As often as necessary to keep the animals clean and dry
Which of the following is NOT a symptom of dehydration? A- shaggy fur B- skin tenting C- sunken eyes D- smooth fur
D- smooth fur
Laboratory animals are provided with environmental enrichment in order to:
Reduce boredom
Which of these devices should be considered a form of environmental enrichment? A- automated watering systems B- indirect bedding C- resting boards D- pelleted feed
C- resting boards
Which of the following is NOT a reason to keep clear, accurate records of daily room checks?
A- inspectors, such as AAALAC site visitors or USDA inspectors, may ask for them
B- if there is a problem with the heating or ventilation, records can help pinpoint when the problem began
C- animal rooms must be checked every day and paperwork is used to monitor the checks
D- the AVMA requires daily room check records to be kept
D- the AVMA requires daily room check records to be kept
Taurine is an essential amino acid for what animal?
Cats
Proteins are made from which molecules?
Amino acids
What is the most important nutrient?
Water
Which is NOT a function of fat? A- to store energy B- to store fat-soluble vitamins C- to help build certain hormones D- to provide essential amino acids
D- to provide essential amino acids
Which animal must have vitamin C supplied in its diet?
Guinea pig
Skin tenting is a sign of what problem?
Dehydration
What type of diet, when fed to rodents, does not wear down their teeth and may allow their teeth to overgrow?
Ground feed
Newborn _____ do not have brown fat and are therefore highly susceptible to hypothermia
Pigs
After the animal room has entered its dark cycle, the lights should not be turned on because:
Physiological changes can occur in the animals
Which environmental conditions are likely to cause the most rapid breakdown of vitamin C in stored feed?
Hot and humid
