UNIT 9 MOUNTING AND LABELLING

Question 1

Process that involves the use of a medium and a
coverslip to facilitate the ease of handling and storage
of the slide and to prevent damage to the section

Answer 1

mounting

Question 2

Mounting is important because the mounting medium
will improve the

Answer 2

refractive index

Question 3

Syrupy fluid applied between the section and the
coverslip after staining, setting the section firmly
preventing the movement of the coverslip.

Answer 3

mounting medium

Question 4

★ Protects the stained section from scratches.
★ Facilitate easy handling.
★ Allows permanent keeping and storage.
★ Helps to prevent the distortion of image during
microscopic examination

Answer 4

benefits of mounting

Question 5

The actual specimen (ex. kidney or liver) can
be disposed 15-30 days after the results
were released.

Answer 5

TRUE

Question 6

Refractive index near to that of the glass

Answer 6

1.518

Question 7

Should be freely miscible with xylene and toluene.

Answer 7

TRUE

Question 8

Sets hard and produces permanent mounting of
sections.

Answer 8

TRUE

Question 9

Designed to mount
water-miscible
preparations directly from
water in cases where the
stain is removed or
decolorized with alcohol
or xylene, or for
metachromatic staining
of amyloid.

Answer 9

aqueous mounting

Question 10

Used for preparations that
have been dehydrated and
cleared in xylene or
toluene, and are
recommended for the
majority of staining
methods.

Answer 10

resinous mounting media

Question 11

aqueous is usually made up of:

Answer 11

GGGSP
1. glycerol
2. gelatin
3. glycerin jelly or gum arabic
4. sugar
5. preservative solution

Question 12

Temporary medium.
○ We may simply use
water if the slide is
immediately

Answer 12

water

Question 13

Transparent and
colorless resin that
adheres to glass;

Answer 13

canada basalm

Question 14

Preservative actions;
○ Set hard;

Answer 14

glycerin

Question 15

Keeps sections for
years after ringing.

Answer 15

glycerin

Question 16

Good preservative
for the nuclear detail
but is not commonly
used because it’s
expensive.

Answer 16

glycerin

Question 17

It quickly hardens
compared to other
media.

Answer 17

glycerin

Question 18

Dissolved Gum
Arabic;

Answer 18

farrant’s medium

Question 19

Takes longer time to
harden and requires
a ringing.

Answer 19

farrants medium

Question 20

acts like a sealant that would seal the edges of the tissue slide

Answer 20

ringing

Question 21

Used for methylene
blue stained nerve preparations

Answer 21

apathys medium

Question 22

Sets hard (compared
to the farrant’s) and
does not require
ringing.

Answer 22

apathys medium

Question 23

Recommended for
mounting frozen
sections.

Answer 23

brun’s fluid

Question 24

Darkens with age and
oxidizes xylene to
become acidic;
■ Drawback:
becomes
brownish over
time.

Answer 24

canada basalm

Question 25

Recommended for
while mounts and
thick sections
○ This was commonly
used in the past until
we started developing.

Answer 25

canada basalm

Question 26

For small tissue
shrinkage produced
upon drying;
○ Dries rapidly.

Answer 26

DPX (dibutylphthalate polystyrene xylene)

Question 27

Synthetic mixture in
xylene;
○ Dries quickly without
retraction.

Answer 27

XAM

Question 28

the
edges lack
mounting media
that’s why the
mounted section
shrinks.

Answer 28

retraction

Question 29

Process of sealing the margins of the coverslip to
prevent the escape of fluid or semi-fluid mounts and
evaporation of mountant, to immobilize the coverslip,
and to prevent sticking of the slides upon storage

Answer 29

ringing

Question 30

ringing is not commonly used for_______ mounting media

Answer 30

resinous

Question 31

Made up of two parts paraffin wax mixed with 4-9 parts powdered
colophonium resin, heated and
filtered.

Answer 31

kronig cement

Question 32

Cellulose adhesives

Answer 32

durofix