unit 8 Flashcards
DNA, protein synthesis, and genetic engineering
what are chromosomes made out of?
DNA pieces coiled around proteins called histones
DNA is what kind of molecule and what is it’s function?
DNA is a macromolecule (nucleic acid) and its function is to share genetic information
how many and what kind of DNA are there?
DNA and RNA
nucleotides are made up of 3 parts, what are they?
sugar (5-carbon), phosphate, nitrogen base
where does DNA nucleotide stay?
stays inside the nucleus
where does RNA nucleotide stay?
stays in and outside nucleus
DNA nucleotides are made up of–
a deoxyribose sugar, phosphate, and 4 nitrogen base
what are the four nitrogen bases DNA nucleotides are made up of?
adenine, guanine, cytosine, thymine
what is the shape of DNA?
two strands connected, shape of a twisted ladder-double helix
what makes up the sides of the rails of the double helix of DNA?
sugar and phosphate
true or false: DNA has directionality: read from 5’ to 3’
true
the strands of DNA run in what direction?
they run in opposite directions, antiparallel
the 5’ (5 prime) end is at the–
phosphate
the 3’ (3 prime) end is at the–
sugar
DNA codes for–
proteins
Erwin Chargaff–
discovers the rules of base pairing: A-T, G-C
Rosalind Franklin & Maurice Wilkins–
determined that the DNA molecule was a coiled spiral, they used a technique called x-ray diffraction
James Watson & Francis Crick–
determined the exact structure of DNA, received the Nobel prize in 1962
DNA replication–
-occurs in the nucleus of eukaryotes and in the cytoplasm of prokaryotes
-occurs during interphase (S phase) in eukaryotes
-there are approximately 6 billion pairs of nucleotides in human body cells (3 billion in haploid)
-it takes about six hours to copy all your DNA
-a DNA molecule produces 2 identical copies of iteslf
steps in DNA replication–
- enzymes unwind and unzip the DNA double helix
- the enzyme DNA polymerase brings complementary nucleotides and attaches them to the matching nucleotides in the open strands
why is DNA replication called semiconservative?
it is called that because two copies of the original DNA molecule are produced, each copy replicating the info from one half of the original DNA molecule.
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