Unit 5 Diagnosis of infectious Diseases Flashcards

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1
Q

what are 3 reasons that prompt and accurate identificaiton of infectious agents by the lab is needed

A

provide patient with appropriate and effective therapy in a timely fashion
avoid delay in providing therapy and to avoid providing ineffective/harmful therapy
to help reduce risk of further spread of the infection (and hence reduce morbidity and mortality)

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2
Q

what are the three phases for laboratory diagnosis of infectious diseases

A

pre-analytic phase
analytic phase
post-analytic phase

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3
Q

what happens in the pre-analytic phase of laboratory diagnosis of infectious diseases

A

order placed
order transfered to lab
identifying information entered
specimen obtained

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4
Q

what occurs in the analytic phase of laboratory diagnosis of infectious disease

A

specimen analyzed

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5
Q

what happens in the post-analytic phase of laboratory diagnosis of infectious disease

A

report generated
result conveyed to clinician
data interpreted
clinical response to result

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6
Q

which phase are the most mistakes made

A

pre-analytical phase

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7
Q

what aer the five major steps of how patient specimens are processed for conventional testing

A
inoculation
incubation
isolation
inspection
identification
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8
Q

who out of the health care team is the first to be aware of S&S of infection in a patient

A

nurse

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9
Q

what happens in step 1 of proper specimen collection and handling

A

provider places laboratory order in the EHR

order is electronically transmitted to the LIS

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10
Q

what happens in step 2 of proper specimen collection and handling

A

nurses open the worklist in the EHR to view pending collections

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11
Q

what happens in step 3 of proper specimen collection and handling

A

when nurse is ready to collect the specimens he/she clicks the “print label” task in the EHR. The rpinted label contains patient identifiers and collecion information
nurse brings collection materials into the patients room

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12
Q

what happens in stage 4 of proper specimen collection

A

nurse scans patients wristand against he specimen labels to ensure a match. The EHR records successful positive patient identification or alerst the provider of a mistmatch
specimens are collected and sent to the laboratory

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13
Q

what is collected from the patient in serology

A

paired sera

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14
Q

what do analysts look for in serology

A

a significant rise of at least 4 fold increase in antibody titre

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15
Q

true or false it is important to collect a specimen before the patient receives antimicrobials

A

true

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16
Q

why is it important to collect the specimen before a patient receives antimicrobials

A

otherwise the causative agent could be killed by the drugs to prevent its isolation and identification by the lab

17
Q

when is the most opportune time to collect sputum

A

when a paitent first wakes up and coughs

18
Q

true or false different time limits may be applied to specimens

A

true

19
Q

what are the three categories used in clinical microbiology for identification

A

phenotypic methods
genotypic methods
immunological methods

20
Q

what is culture and sensitivity tests used to determine

A

whether the test organism is sensitive to antibiotics

21
Q

regarding phenotypic methods what two methods of examination are used

A

microscopic examination

macroscopic examination

22
Q

what is microscopic examination

A

gram stain
acid-fast stain
electron microscopy

23
Q

what is macroscopic examination

A

characterisitics of bacterial colonies on an agar plate such as their growth patterns, texture, colours and shape

24
Q

what happens in the phenotypic method of isolation

A

must isolate the disease causing agent before testing is done on them

25
Q

what happens after isolation

A

culturing or growing of microbes to sufficient numbers