UNIT 3-ENZYMES🤎 Flashcards

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1
Q

define an enzyme using keywords (3)

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2
Q

differentiate between intracellular and extra cellular enzymes

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3
Q

explain the lock and key hypothesis and induced fit hypothesis in detail using keywords (active site, enzyme-substrate complex, activation energy, specificity)

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4
Q

define activation energy, draw two graphs to show the effect of enzymes on activation energy

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5
Q

explain two ways one can measure the progress of an enzyme-catalysed reaction, give examples for each and draw graphs if necessary

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5
Q

explain the effect of temperature on the rate of enzyme-catalysed reaction in detail, use a graph if necessary

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5
Q

explain the effect of PH on the rate of enzyme-catalysed reaction in detail, use a graph if necessary, relate the use of buffer solutions to your explanation

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6
Q

explain the effect of enzyme concentration on the rate of enzyme-catalysed reaction in detail, use a graph if necessary

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7
Q

explain the effect of substrate concentration on the rate of enzyme-catalysed reaction in detail, use a graph if necessary, define V max

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8
Q

use a graph to explain the concepts of V max and Km then relate the two concepts to enzyme affinity

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9
Q

explain the effect of affinity on substrate concentration using a graph

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10
Q

state two types of enzyme inhibitors, state whether they are reversible or non-reversible

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11
Q

use a graph to explain the effect of competitive inhibition on enzyme activity

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12
Q

use a graph to explain the effect of non-competitive inhibition on enzyme activity

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13
Q

explain the concept of end-product inhibition (not on syllabus, just for understanding)

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13
Q

what is enzyme immobilisation? explain the difference in enzyme activity between immobilised enzymes and enzymes free in solution

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13
Q

explain two advantages of using immobilised enzymes

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