UNIT 2.1 SPECIMEN Flashcards
Like routine histopath
Rush Frozen Section
smears
Cytology
S-2020-3DMT-0001 In Routine Histopath, The last four numbers indicate the
number of specimens
processed
Commonly used for Hirschsprung disease
Rush Frozen Section
tissue to formalin ratio
1:10 or 1:20
smears
Cytology
Special for renal and skin biopsies
Immunofluorescence
Received from surgical resection
Rush Frozen Section
Special for renal and skin biopsies
Immunofluorescence
Completion of fixation must be done within
24-48
hours
putrefaction meaning
Tissue has already decayed
Smear is done by the
doctor who collected the specimen
If you want to see ultramicroscopic details of the cell
Electron Microscopy
Same fixative as immunofluorescence
Immunostaining
transport time should be
Within 1 hour of collection
Specimen is unfixed
Rush Frozen Section
Saline or liquid nitrogen fixative
Immunofluorescence
Same fixative as immunofluorescence
Immunostaining
Saline or liquid nitrogen fixative
Immunofluorescence
Smear is done by
doctor who collected the specimen
Uses antibodies to detect specific locations in the tissue
- Immunofluorescence
- Immunostaining
Rapid diagnosis
Rush Frozen Section
Frozen using liquefied carbon dioxide or liquid nitrogen
Rush Frozen Section
Tissue/Organ
Routine Histopath
Uses antibodies to detect specific locations in the tissue
- Immunofluorescence
- Immunostaining