UNIT 2.1 RECEIVING: LOGGING AND NUMBERING and ACCESSIONING/NUMBERING Flashcards
RECEIVING: LOGGING AND NUMBERING:
- Verify information on the request form
- Verification of the Specimen Label
- Enter all data in the logbook/LIS
- Accession/Number the specimen
ACCESSIONING/
NUMBERING purpose
Identify specimen without writing the name of the
patient
Initial assessment of the specimen occurs in the
reception area
ALL SPECIMENS MUST BE DELIVERED TO THE LABORATORY IMMEDIATELY (WITHIN 1HOUR MAXIMUM) WITH A
COMPLETED REQUISITION FORM
ALL REQUISITION FORMS MUST BE LABELED WITH THE CORRECT INFORMATION OF THE PATIENT AS
FOLLOWS:
- Name of Patient.
- Age, Date of Birth, Sex.
- Medical Record Number
- Date and Time of Collection
- Type of Specimen (anatomic site)
- Location of the Patient
- Clinical history of the patient
- Pre/post operation diagnosis
- The requesting physician’s name.
- Physician or authorized person’s signature on the request form.
- Name and address of physician (for referral laboratories)
. Specimen container must be labeled with the correct
information of the patient as follows:
1.Full patient’s name.
2.Medical record number.
3.Age and sex of the patient.
4.Type of specimen (Anatomic site).
5.Date and time of collection.
6.Location of the patient
specimens that accompany the requisition form should be
identified by at least how many patient identifiers
2
two identifiers in specimen:
Medical Record Number (MRN) and
pathology number
S13-000 what is 000?
pangilan na specimen this year
The case specimen number if there are multiple
specimens for the same patient case are labeled
alphabetically as A, B, C etc
S13-000 what is 13?
year
CASE ACCESSION NUMBER
S13-000 what is S?
What type of specimen?
if there are
multiple blocks on the same specimen, use
specimen block number
(labeled
numerically A1, A2, A3 etc.)
if there are multiple slides cut from a
single block
block slide number (numerically as 1,2,3 etc.)
Stained Slides will be identified by two numbers :
medical record number and Pathology number
All slides other than those stained by ________ and _________
will bear the name of the stain
hematoxylin and eosin
it has bacterial contamination. The
bacteria destroyed the cells
Putrefaction
maintains the records of the number of blocks,
slides, and stains prepared and it is capable of demonstrating
volumes for any given time
QUADRAMED
If you are going to send specimens out, it will not be accepted
by the referral pathology laboratory without __________
histopath report
the cell is destroyed because of the natural process of decomposition, or because of the enzymes
inside the ce
autolysis
ALL SPECIMENS FROM OUTSIDE THE HOSPITAL EXCEPT THOSE FROM GOVERNMENT HOSPITALS Must have the ____________ before they are accepted for processing
approval of the hospital administration and
consultant on duty
. All ____________________ must have their laboratory tests entered and collected in the computer by the attending physician
In-patients for histopathological diagnosis
REQUESTS FOR FROZEN SECTION REQUIRE _______ AHEAD OF THE OPERATION.
24 HOUR-NOTICE
must
be duly completed and submitted with the specimen
Histopathology requisition form (Lab 1-A)
Accessioning of collected specimen is carried out in the _____________ by the technologist(s
laboratory reception area
Specimen should be immersed in fixative (10% buffered formalin) within _______ of the biopsy or
resection procedure.
1 hour
All specimens must be received fixed in 10% neutral buffered formalin EXCEPT for the following:
- FROZEN SECTION
- IMMUNOFLUORESCENCE
- ELECTRON MICROSCOPY
IMMUNOFLUORESCENCE fixative
Either in normal saline solution (NSS) or liquid nitrogen
ELECTRON MICROSCOPY fixative
2% glutaraldehyde solution in the cold after excision
FOR RENAL BIOPSIES, Please call __________ before the procedure
15 minutes
FOR RENAL BIOPSIES, sample should be immersed in
saline
RENAL BIOPSIES
The specimen will be examined under the dissecting microscope for the presence of _________ to determine if the specimen is adequate, then divided into 3 parts:
- glomeruli
○ (1) frozen for immunofluorescence
○ (1) immersed in glutaraldehyde for EM study
○ (1) fixed in formalin for paraffin processing
RENAL BIOPSIES
Alternatively, __ separate samples could be sent to the Histopathology
laboratory.
3
RENAL BIOPSIES
These specimens should be sent to the lab within _______ after the renal biopsy procedure.
one hour
RENAL BIOPSIES
in renal biopsy, The specimen in saline should be immediately snap frozen in _________ for
further frozen sectioning and immunofluorescence staining.
liquid nitrogen
TESTICULAR BIOPSIES fixative
BOUIN’S
FLUID
ELECTRON MICROSCOPY SAMPLES:
4%
GLUTARALDEHYDE SOLUTION AND REFRIGERATE.
thread used for sewing; used for differentiation of
positions
Sutures
FOR BREAST LUMP, THE SURGICAL MARGINS
SHOULD BE ORIENTED BY ________.
SUTURES
FOR MASTECTOMY, THE _______ SHOULD
BE INDICATED BY SUTURES.
APICAL NODES
IDENTIFIED BY THE
SURGEON AND REMOVED WILL BE SUBMITTED TO THE
LABORATORY IN A SEPARATE CONTAINER, WITH A
REQUISITION.
Sentinel lymph node/nodes
Superior margin to be indicated by a _________ in case
of simple mastectomy (without axillary dissection)
separate suture
the first lymph node to be affected by
cancer
Sentinel lymph node
This will ensure the integrity of the lymph node capsules
and also assess the tumor spread beyond the lymph node
capsule.
including Perinodal fat
Known infective specimens should be clearly marked with
red
marker.
The proximal or distal excised margins should
be oriented by a suture.
BOWEL RESECTION
SKIN BIOPSIES INTENDED FOR
IMMUNOFLUORESCENT ARE STORED IN THE ______ FOR IMMUNOFLUORESCENCE STUDIES.
DEEP FREEZER (-70 c)
Surgical specimen to be “Exempt from Pathology Examination” as approved by the Chairperson Department of Pathology in conjunction with the hospital administration and medical staff departments, are
- Foreign bodies (e.g. bullets, medical devices, IUD, valve rings).
- Hair, finger nails, toe nails removed
for cosmetic reason. - Teeth, dental appliances.
- Lens (i.e. cataract).
- Calculi.
- Nasal septum cartilage and bone (soft tissue is submitted for microscopic
examination). - Foreskin from circumcision (10 years old and younger).
- Mammary implant
- Skin from face-lifts
- Orthopedic materials
___________ INTENDED FOR
IMMUNOFLUORESCENT ARE RECEIVED FROZEN FROM
THE DERMATOLOGY DEPARTMENT
SKIN BIOPSIES
Tiny and small biopsies from ___________ and ___________ are
normally submitted for processing on the same day, if received before
the cut-off time i.e., before 2.00 P.M. and if they appear to be fixed.
Endoscopy and dermatology
Specimens are usually kept at room temperature for at__________ after the official report is released and then disposed according to Hospital and Municipality protocol on proper specimen disposal
least two weeks
Referral slides/blocks must be accompanied by the official original pathology report with the following information:
- Patient’s name
- Medical Record Number
- Hospital / clinic pathology number
- Number of slides/ blocks
Specimens accepted for “Gross Description
Only”
- Torn Meniscus.
- Varicose veins.
- Bone fragments from non-pathologic fracture.
- Ear cartilage.
- Fetus.
