Instrumentation in Histopathology (Microtome Knives)

Question 1

BEVEL ANGLE

Answer 1

27-32°

Question 2

Are brittle and expensive, but
very durable

Answer 2

Diamond Knives

Question 3

Have both sides straight

Answer 3

PLANE-WEDGE KNIFE

Question 4

Recommended for cutting paraffin embedded sections on a
rotary microtome

Answer 4

BICONCAVE KNIFE

Question 5

It is the result of the clearance angle and the upper bevel
angle of the knife or blade.

Answer 5

CUTTING ANGLE

Question 6

Angle of the edges of the wedge knife

Answer 6

WEDGE ANGLE

Question 7

Used for trimming and semithin sectioning of tissue blocks
for electron microscopy.

Answer 7

Glass Knives

Question 8

One side of the knife is flat while the other is concave

Answer 8

PLANE-CONCAVE KNIFE

Question 9

This is the body of the knife or blade.

Answer 9

WEDGE ANGLE

Question 10

kind of slide used in In HistoPath laboratory

Answer 10

frost-ended slides

Question 11

Can be used to remove excess paraffin so that only the
tissue is on the slide

Answer 11

MICROWAVE

Question 12

Used for hard sharpening

Answer 12

Honing

Question 13

kind of slide used In the Hematology lab

Answer 13

the normal slides are used.

Question 14

in floatation water bath, A small amount of _____ may be added to water to reduce surface tension and allow section to flatten out.

Answer 14

detergent

Question 15

CLEARANCE ANGLE

Answer 15

5-10°

Question 16

Dependent on the tilt or knife holder

Answer 16

CLEARANCE ANGLE

Question 17

Should be prepared and
stored in dust-free boxes with
lids, just before use, to avoid
contamination.

Answer 17

Glass Knives

Question 18

Floatation Water Bath temperature

Answer 18

About 10°C below the melting point of the paraffin wax.

Question 19

Used to cut any type of resin
block for electron microscopy

Answer 19

Diamond Knives

Question 20

Recommended for frozen sections or for cutting extremely
hard and tough specimens embedded in paraffin blocks
using a base-sledge type or sliding microtome

Answer 20

PLANE-WEDGE KNIFE

Question 21

The concave sideof the PLANE-CONCAVE KNIFE is used to cut what

Answer 21

paraffin sections

Question 22

Done to remove burrs

Answer 22

Stropping

Question 23

Both are needed for handling sections during cutting and
removing folds and creases during “floating out” in water bath

Answer 23

FORCEPS AND SQUIRREL/CAMEL HAIRBRUSH

Question 24

PLANE-CONCAVE KNIFE length

Answer 24

25 mm

Question 25

They are already mounted in a
metal block designed to fit
directly into the knife holder of
the ultrathin microtome when
purchased

Answer 25

Diamond Knives

Question 26

BICONCAVE KNIFE length

Answer 26

120 mm

Question 27

Angle of the very tip of the knife or blade, between cutting
facets

Answer 27

BEVEL ANGLE

Question 28

90° minus the angle of the upper facet of the knife

Answer 28

RAKE ANGLE

Question 29

Angle between the block face and lower facet of the knife

Answer 29

CLEARANCE ANGLE

Question 30

Commercially prepared

Answer 30

Glass Knives

Question 31

Angle between the block face and upper facet of the knife

Answer 31

CUTTING ANGLE

Question 32

PLANE-WEDGE KNIFE
length

Answer 32

100 mm

Question 33

WEDGE ANGLE

Answer 33

15°

Question 34

DRYING OVEN OR HOT PLATE temperature

Answer 34

melting point of the wax, 2-5 degrees
Celsius above* (*Gregorio’s)

Question 35

The flat side of the PLANE-CONCAVE KNIFE is recommended for cutting what

Answer 35

celloidin-embedded

Question 36

Have a sharp cutting edge that can cut 2-4 µ thick sections
with ease

Answer 36

DISPOSABLE BLADES