Instrumentation in Histopathology (Microtome Knives) Flashcards

1
Q

BEVEL ANGLE

A

27-32°

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2
Q

Are brittle and expensive, but
very durable

A

Diamond Knives

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3
Q

Have both sides straight

A

PLANE-WEDGE KNIFE

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4
Q

Recommended for cutting paraffin embedded sections on a
rotary microtome

A

BICONCAVE KNIFE

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5
Q

It is the result of the clearance angle and the upper bevel
angle of the knife or blade.

A

CUTTING ANGLE

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6
Q

Angle of the edges of the wedge knife

A

WEDGE ANGLE

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7
Q

Used for trimming and semithin sectioning of tissue blocks
for electron microscopy.

A

Glass Knives

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8
Q

One side of the knife is flat while the other is concave

A

PLANE-CONCAVE KNIFE

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9
Q

This is the body of the knife or blade.

A

WEDGE ANGLE

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10
Q

kind of slide used in In HistoPath laboratory

A

frost-ended slides

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11
Q

Can be used to remove excess paraffin so that only the
tissue is on the slide

A

MICROWAVE

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12
Q

Used for hard sharpening

A

Honing

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13
Q

kind of slide used In the Hematology lab

A

the normal slides are used.

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14
Q

in floatation water bath, A small amount of _____ may be added to water to reduce surface tension and allow section to flatten out.

A

detergent

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15
Q

CLEARANCE ANGLE

A

5-10°

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16
Q

Dependent on the tilt or knife holder

A

CLEARANCE ANGLE

17
Q

Should be prepared and
stored in dust-free boxes with
lids, just before use, to avoid
contamination.

A

Glass Knives

18
Q

Floatation Water Bath temperature

A

About 10°C below the melting point of the paraffin wax.

19
Q

Used to cut any type of resin
block for electron microscopy

A

Diamond Knives

20
Q

Recommended for frozen sections or for cutting extremely
hard and tough specimens embedded in paraffin blocks
using a base-sledge type or sliding microtome

A

PLANE-WEDGE KNIFE

21
Q

The concave sideof the PLANE-CONCAVE KNIFE is used to cut what

A

paraffin sections

22
Q

Done to remove burrs

A

Stropping

23
Q

Both are needed for handling sections during cutting and
removing folds and creases during “floating out” in water bath

A

FORCEPS AND SQUIRREL/CAMEL HAIRBRUSH

24
Q

PLANE-CONCAVE KNIFE length

A

25 mm

25
Q

They are already mounted in a
metal block designed to fit
directly into the knife holder of
the ultrathin microtome when
purchased

A

Diamond Knives

26
Q

BICONCAVE KNIFE length

A

120 mm

27
Q

Angle of the very tip of the knife or blade, between cutting
facets

A

BEVEL ANGLE

28
Q

90° minus the angle of the upper facet of the knife

A

RAKE ANGLE

29
Q

Angle between the block face and lower facet of the knife

A

CLEARANCE ANGLE

30
Q

Commercially prepared

A

Glass Knives

31
Q

Angle between the block face and upper facet of the knife

A

CUTTING ANGLE

32
Q

PLANE-WEDGE KNIFE
length

A

100 mm

33
Q

WEDGE ANGLE

A

15°

34
Q

DRYING OVEN OR HOT PLATE temperature

A

melting point of the wax, 2-5 degrees
Celsius above* (*Gregorio’s)

35
Q

The flat side of the PLANE-CONCAVE KNIFE is recommended for cutting what

A

celloidin-embedded

36
Q

Have a sharp cutting edge that can cut 2-4 µ thick sections
with ease

A

DISPOSABLE BLADES