Instrumentation in Histopathology (Tissue Processing)

Question 1

Steps of tissue processing

Answer 1

1. Numbering
2. Fiaxation
3. Decalcification
4. Dehydration
5. Clearing
6. Impregnation
7. Embedding
8. blocking
9. trimming
10. sectioning
11. staining
12. mounting
13. Labelling

Question 2

Where you put or assign a specific accession number

Answer 2

Numbering

Question 3

also known as accessioning

Answer 3

Numbering

Question 4

preservation of tissues

Answer 4

Fixation

Question 5

Only do if the specimen is osseous tissue/bone or the specimen are cartilages

Answer 5

Decalcification

Question 5

Removal of calcium or lime salts

Answer 5

Decalcification

Question 6

Removal of water

Answer 6

Dehydration

Question 6

You need to make the tissue translucent in appearance

Answer 6

Clearing

Question 7

Usage of paraffin to solidify the tissue for cutting

Answer 7

Impregnation

Question 8

You put the tissue and the paraffin together with the mold

Answer 8

Embedding

Question 9

The product of embedding where u produce a paraffin
block

Answer 9

Blocking

Question 10

Because the tissue in the paraffin block will not fit to the microtome to be used for sectioning

Answer 10

Trimming

Question 11

shape used in trimming

Answer 11

truncated pyramid

Question 12

Production of tissue ribbons which will be stuck to the
slide

Answer 12

Sectioning

Question 13

machine used in sectioning

Answer 13

microtome

Question 14

Where you put colors for the identification of cells and
the tissue itself

Answer 14

Staining

Question 15

Where you put the cover slip to protect the slides

Answer 15

Mounting