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Batce lec > UNIT 2 STAINING PROCEDURES > Flashcards

UNIT 2 STAINING PROCEDURES Flashcards

1
Q

More sensitive as compared to
routine stains

A

FLUORESCENT DYES

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2
Q

Cell Wall

A

Dyar Stain

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3
Q

Stains/visualizes the form and shape of the bacterial cell

A

SIMPLE STAINING

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4
Q

stains for specific parts of a bacterial cell

A

SPECIAL STAINING

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5
Q

Bacteria appear as light-colored bodies against a dark background

A

INDIRECT/ RELIEF STAINING/ NEGATIVE STAINING

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6
Q

acidic DYES bind to

A

(+) charged
molecules

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7
Q

Flagella

A

Gray Stain
Leifson Stain

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8
Q

A fluorochrome dye that stains both gram-positive and
gram-negative bacteria, living or dead.

A

Acridine orange

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9
Q

CLASSES OF DYES

Ex. methylene blue, basic fuchsin, crystal violet, safranin, malachite green

A

BASIC DYES

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10
Q

CLASSES OF DYES

Ex. eosin, rose bengal, acid fuchsin

A

ACID DYES

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11
Q

DIFFERENTIAL STAIN examples

A
  • GRAM STAINING
  • ACID-FAST STAINING
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12
Q

“Halo” appearanc

A

INDIRECT/ RELIEF STAINING/ NEGATIVE STAINING

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13
Q

INDIRECT/ RELIEF STAINING/ NEGATIVE STAINING example

A

ndia ink

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14
Q

FLUORESCENT STAINING use:

A
  • acridine orange,
  • rhodamine-auramine (Truant Method for M. tuberculosis)
  • Fluorescein isothiocyanate (FITC), calcofluor white
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15
Q

SIMPLE STAINING examples

A
  • methylene blue
  • basic fuchsin
  • crystal violet
  • safranin
  • malachite green
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16
Q

CLASSES OF DYES

A
  • basic
  • acidic
17
Q

DNA

A

Feulgen Stain

18
Q

acidic dye charge group

A

Anionic (-)

19
Q

Spirochetes

A

Levaditi’s Stain

20
Q

1 stain is used

A

SIMPLE STAINING

21
Q

fluorochrome that binds to chitin in fungal cell walls

A

Calcofluor White

22
Q

Primarily to demonstrate the capsule

A

INDIRECT/ RELIEF STAINING/ NEGATIVE STAINING

23
Q

ACID-FAST STAINING stains

A

○ Primary stain: carbolfuchsin
○ Secondary stain: methylene blue or malachite green

24
Q

Basic dye charge group

A

Cationic (+)

25
Q

Contains a primary and secondary stain

A

DIFFERENTIAL STAIN

26
Q

Endospores

A

Fulton Schaeffer Stain
Dorner Stain
Wirtz and Conklin

27
Q

Used to visualize capsulated bacteria such as Klebsiella
pneumoniae

A

INDIRECT/ RELIEF STAINING/ NEGATIVE STAINING

28
Q

BASIC DYES bind to

A

(-) negative charge cells

29
Q

Metachromatic Granules)

A

Neisser Stain
Albert Stain
LAMB Stain

30
Q

Use of 2 or more dyes

A

DIFFERENTIAL STAIN

31
Q

Gram STAINING stains:

A

○ Primary stain: crystal violet
○ Secondary stain: safranin

Batce lec (13 decks)

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