UNIT 2 GRAM STAINING Flashcards
1
Q
Fundamental Staining Method in Bacteriology
A
GRAM STAINING
2
Q
Developed by
A
Hans Christian Gram
2
Q
involves the use of:
A
○ Primary stain
○ Mordant
○ Decolorizer
○ Secondary stain
2
Q
determines the Gram-staining characteristics of a species
A
Cell wall structure
3
Q
PRIMARY STAIN
A
CRYSTAL VIOLET
3
Q
MORDANT
A
GRAM’S IODINE
4
Q
DECOLORIZER
A
95% ETHANOL or
ACETONE-ALCOHOL
5
Q
SECONDARY STAIN
A
SAFRANIN O or
CARBOL FUCHSIN
5
Q
95% ETHANOL or
ACETONE-ALCOHOL in gram - color
A
colorless
5
Q
SAFRANIN O or
CARBOL FUCHSIN in gram - color
A
Red/dark
pink
6
Q
bacteria appear pink
under the light
microscope
A
GRAM-NEGATIVE
7
Q
retain the primary crystal
violet stain during the
decolorizing treatment
and appear purple. (blue
to purple)
A
GRAM-POSITIVE
