UNIT 1 RED BLOOD CELLS

Question 1

Drabkin reagent is a weak solution of

Answer 1

potassium cyanide and
potassium ferricyanide

Question 2

provide stable measurements for internal uality control of automated blood cell analyzers.

Answer 2

RBC indices

Question 3

RED BLOOD CELL INDICES To compute, may use the three numerical results:

Answer 3

• RBC count
• HgB
• HcT

Question 4

principle still used to count RBCs in many automated blood cell analyzers.

Answer 4

Coulter Principle of direct current electrical
impedance

Question 5

Also called packed cell volume (PCV)

Answer 5

HEMATOCRIT (Hct)

Question 6

how many RBCs per uL

Answer 6

4 to 6 million/ uL

Question 7

percent of saline used in RBC count

Answer 7

0.85%

Question 8

Spectrophotometer measures the absorbance or color intensity of the solution at what wavelenght

Answer 8

540 nm wavelength

Question 9

Normal ratio of Hematocrit

Answer 9

50%

Question 10

ome automated blood cell analyzers replace cyanmethemoglobin
with a formulation of the ionic surfactant (detergent) ___________ to reduce environmental cyanide.

Answer 10

sodium lauryl sulfate or sodium dodecyl
sulfate

Question 11

expresses the degree of variation in RBC volume

Answer 11

RBC DISTRIBUTION WIDTH (RDW)

Question 12

Hemoglobin measurement relies on what reagent?

Answer 12

Drabkin reagent

Question 13

RETICULOCYTES color

Answer 13

slightly blue-gray

Question 14

Glass counting chamber

Answer 14

hemocytometer

Question 15

after adding drabkin reagent to whole blood,

Answer 15

hemoglobin is converted to stable cyanmethemoglobin (hemiglobincyanide)

Question 16

Supravital stains:

Answer 16

Brilliant Cresyl Blue (BCB) and New Methylene Blue (NMB)

Question 17

Indicative of bone marrow regeneration during blood loss and certain anemias

Answer 17

Reticulocytes

Question 18

absorbed by live cells

Answer 18

supravital stains

Question 19

rbc size

Answer 19

7 to 8 um in diameter

Question 20

First patented electronic counter

Answer 20

Joseph and
Wallace Coulter
(1953

Question 21

is excluded from the hematocrit
determination

Answer 21

Buffy Coats

Question 22

Ratio of the volume of packed RBCs to the volume of
whole blood

Answer 22

HEMATOCRIT (Hct)

Question 23

reflects RBC staining intensity and amount of central pallor

Answer 23

MEAN CELL HEMOGLOBIN CONCENTRATION
(MCHC)

Question 24

pippete used routinely until the
advent of automation

Answer 24

Thoma pipette

Question 25

occupies one third of their center reflecting their biconcavity

Answer 25

zone of pallor

Question 26

Extreme RBC volume variability

Answer 26

anisocytosis

Question 27

expresses the mass of hemoglobin per cell

Answer 27

MEAN CELL HEMOGLOBIN (MCH)

Question 28

polychromatic (polychromatophilic) erythrocytes

Answer 28

RETICULOCYTES

Question 29

dillution typical for RBC counts

Answer 29

1:200 dilution

Question 30

0.5% to 2.5% of RBCs that exceed the 7- to 8-mm average diameter

Answer 30

RETICULOCYTES

Question 31

methylene blue dyes that are used to differentiate and count these young RBCs

Answer 31

Nucleic Acid Stains or Vital Stains (supravital)