UNIT 1 ADVANCE HEMATOLOGY PROCEDURES

Question 1

identifies and quantifies populations of
cells in a heterogeneous sample - usually blood, bone marrow
or lymph.

Answer 1

FLOW CYTOMETRY

Question 2

These devices are automated clinical blood cell
analyzers that generate the quantitative
parameters of the CBC through application of
electrical impedance and laser or light beam
interruption.

Answer 2

FLOW CYTOMETERS

Question 3

are collected and stained to analyze nucleated cells that are the immature precursors to blood cells.

Answer 3

Bone marrow aspirates and biopsy specimens

Question 4

deals with chromosomes also known as karyotyping used for diagnosis of oncologic and hematologic
disorders

Answer 4

CYTOGENETICS

Question 5

Both qualitative and quantitative flow cytometers are employed to analyze cell populations by:

Answer 5

• measuring the effects of individual cells on laser light
• immunophenotyping

Question 6

are reviewed by
medical laboratory scientists, clinical pathologists,
and hematologists for morphologic abnormalities,
high or low bone marrow cell concentration, and
inappropriate cell line distributions.

Answer 6

Wright-stained aspirate smears

Question 7

precursors to RBCs

Answer 7

Erythroid series cells

Question 8

enhanced by hematoxylin and
eosin (H&E) staining, may reveal abnormalities in
bone marrow architecture indicating leukemia, bone
marrow failure, or one of a host of additional
hematologic disorders.

Answer 8

Biopsy specimens

Question 9

primarily defines a large set of
the techniques that operate either with the entire genome or
with specific targeted DNA sequences

Answer 9

MOLECULAR CYTOGENETICS

Question 10

mature to form bands and
neutrophils, eosinophils, and basophils

Answer 10

Myeloid series cells

Question 11

produce platelets

Answer 11

Megakaryocytes

Question 12

may occasionally be employed to
differentiate abnormal myeloid, erythroid, and lymphoid
cells

Answer 12

Cytochemical stains

Question 13

Cytochemical stains:

Answer 13

■ myeloperoxidase
■ Sudan Black B
■ nonspecific and specific esterase
■ periodic acid-Schiff
■ tartrate-resistant acid phosphatase
■ alkaline phosphatase

Question 14

= types of antigens present on the
cell’s surface, nucleus, or cytoplasm that helps identify the
lineage of cells using antibodies

Answer 14

IMMUNOPHENOTYPING

Question 15

cytometers that are
mechanically simpler but technically more
demanding.

Answer 15

Qualitative laser-based flow cytometers