UNIT 1 - Lecture 7: Intro to Leukocytes Flashcards
What do the following stand for:
WBC
PMN
MNP
CNP
WBC = white blood cell (count)
PMN = polymorphonuclear cells = NPs
MNP = marginating NP pool
CNP = circulating NP pool
How is WBC count expressed?
/ µL
What does a differential leukocyte count determine?
relative #s via %s of the different WBCs
What # should not be interpreted for WBC counts?
%
Should convert to absolute # by multiplying the total WBC
What spp need a manual leukocyte count and what is used to do so?
Avian/Exotics due to nRBCs
Using a hemocytometer
What are challenges to using an impedance counter?
Rmt RBCs, cat platelets
Microscopic evaluation of WBCs should _____ be part of the CBC.
always
What does microscopic evaluation of WBCs identify?
Morphologic changes with pathologic significance
nRBCs vs. WBC
Immature RBCs with nuclei can artificially _____ WBC.
increase
How do you report nRBCs?
nRBC/100 WBC
What do you call an increase in [WBC}?
leukocytosis
What do you call a decrease in [WBC]?
leukopenia
What is a granulocyte? What cells are granulocytes?
Leukocyte with cytoplasmic granules that may or may not be visible;
Have a segmented nucleus;
Neutrophils, eosinophils, basophils
What is the major function of neutrophils?
First line of defense against pathogenic bacteria
What is the most numerous leukocyte in health?
neutrophil
What spp have neutrophils in health?
Dogs, cats, horses, primates
What are the 2 main characteristics of a mature neutrophil?
Segmented nucleus (condensed chromatin), neutral staining cytoplasm
What are 3 main characteristics of immature neutrophils?
Non-segmented nucleus
More basophilic staining cytoplasm (more RNA)
Slightly larger cells
What is Pelger-Huet anomaly and what can it be mistaken for?
Inherited blood condition;
Nuclei of several types of WBC have hyposegmentation with band or peanut shape with mature nuclear chromatin;
Can be mistaken for bands leading to misdiagnosis of inflammation and/or infections
What is the appearance of heterophils?
Cytoplasm contains numerous red-orange oval, needle, or rice-shaped granules
What species have heterophils? Which of these lack myeloperoxidase?
Birds, reptiles, amphibians, rabbits, guinea pigs;
Birds, reptiles, amphibians lack myeloperoxidase
What is the major function of monocytes?
2nd line of defense for infections, immunologic
What happens to monocyte structure when they are in EDTA?
Often form vacuoles
What is the nucleus appearance of monocytes?
Can be variable;
Segmented, blocky/oval, kidney-bean
