Unexpected blood group antibody identification

Question 1

Homozygous expression required to be ruled out for the following antigens

Answer 1

D, C/c, E/e, Fya/b, Jka/b, M/N, S/s, k

Question 2

Homozygous or 2x heterozygous expression required to rule of this antigen

Answer 2

K antigen

Question 3

What gels are used to identify unexpected antibodies

Answer 3

AHG gel (green label)
NaCl gel (red label)

Question 4

What types of screening cells are used?

Answer 4

1. LISS - (white caps)
2. Enzyme treated (red caps)

Question 5

What does enzyme treatment do?

Answer 5

Enzymes cleaves MNS enzymes and Duffy (Fya/b) antigens from cell surface so they cannot react in enzyme-treated cells.
The other antigens reactivity is enhanced as a result.

Question 6

Identifying unexpected antibody set-up

Answer 6

Need 2x AHG gel (green) and NaCl gel (red).
Label with cell 1-11 + positive control.
1. Add 50uL of screening cell into each microtubule.
2. In positive control add 50uL of a RhD+ screening cell.
3. Add 25uL of test plasma into all microtubules (except control)
4. In +ve control add 25uL of weak Anti-D reagent.
5. Incubate at 37C for 15-20mins
6. Centrifuge for 10mins.