U3A2: 5D agricultural applications of CRISPR-Cas9

Question 1

why are CRISPR-Cas9 technologies better than its alternatives?

Answer 1

• more precise
• more affordable

Question 2

potential CRISPR-Cas9 applications for production enhancement

Answer 2

• longer shelf life
• improve nutritional value

Question 3

potential CRISPR-Cas9 applications for plant organelles

Answer 3

• increase chloroplast efficiency
• adjust stomata to improve carbon dioxide uptake
• improve light capture in chloroplasts

Question 4

potential CRISPR-Cas9 applications for chemical resistance

Answer 4

• greater herbicide resistance
• greater pesticide resistance

Question 5

potential CRISPR-Cas9 applications for disease resistance

Answer 5

• immunity against viruses
• immunity against bacteria

Question 6

potential CRISPR-Cas9 applications for weather resistance

Answer 6

• frost tolerance
• heat stress tolerance
• drought tolerance

Question 7

potential CRISPR-Cas9 applications for the Calvin cycle

Answer 7

• modify activity of other Calvin cycle enzymes
• modify Rubisco’s activity
• change C3 plants to C4 or CAM
• introduce alternative routes to photorespiration