U3A1: 4A endonucleases, ligases and polymerases Flashcards
1
Q
role of endonuclease
A
cut DNA by splitting the phosphodiester bond of the sugar-phosphate backbone.
2
Q
circular DNA v linear DNA
A
circular DNA: no. fragments = no. recognition sites
linear DNA: no. fragments = no. recognition sites +1
3
Q
role of ligase
A
joins two fragments of DNA or RNA together. ligase can join both sticky and blunt ends.
4
Q
role of polymerase
A
adds nucleotides to DNA or RNA.
5
Q
primary role of RNA polymerase
A
transcription of genes
6
Q
primary role of DNA polymerase
A
replication/amplification of DNA
