U1 LEC: INTRO TO MYCOLOGY (PART 1) Flashcards
Discipline of biology that deals with, describes a enormous group of organisms denominated fungi
Mycology
Other term for fungal infection
Mycoses
Highlights the productive or beneficial effects and applications of this field of science, particularly in the area of agriculture, biotechnology and environmental biology
Good Mycology
Examples of good mycology
- Fermentation
- Saccharomyces cerevisae (Bakerβs yeast)
- Penicllin (antibiotic)
This implicates that fungi are a cause of multiple phenomena that have deleterious effect on environment and health
Bad Mycology
under Good Mycology
- Transformation and recycling of dead material
- Recycling of carbon and other minerals
- Provide nutrients to the plants
- Important Biotechnological tool (yeast)
- Source of secondary metabolites for production of antibiotics and immunosuppresive drugs (Cyclosporin A)
These are significant phytopathogens, causing life threatening disease in patients with risk factors.
Bad Mycology
Main eukaryotic models in genetics, molecular biology, cell biology, biochemistry and metabolism
Saccharomyces cerevisiae
Fungi used in biological research
- Saccharomyces cerevisiae
- Schizosaccharomyces pombe
Key organism in understanding the mechanism of regulation of the cell cycle
Schizosaccharomyces pombe
Distinct difference of eukaryotic from prokaryotic
presence of nuclear membrane
Non motile, eukaryotic organisms, can be single celled or usually very complex multicellular organisms
Fungi
Fungi is a diverse group made up of?
- classic pathogens
- environmental saprobes
- parasitic spore-producing eukaryotic organisms that lack chlorophyll
Eukaryotic organisms that lack chlorophyll
Achlorophyllous
T/F: Fungi reside in nature and found in any habitat, more common in land than in water.
True
Fungi are ______________ aerobes
obligate/facultative
T/F: Facultative aerobes may or may not require Oxygen.
True
Fungi may live as what type of organisms?
- heterotrophs
- saprotrophs
- parasitic
- chemotropic
This type of organisms feed on other living organisms for nutrients.
Heterotrophs
This type of organisms feed on dead organelles.
Saprotrophs
This type of organisms are dependent on host.
Parasitic
This type of organisms secretes enzymes that degrade a wide range of organic substrates into soluble nutrients which are then transported into the cell through passive absorption or active transport.
Chemotrophic
T/F: Chemotrophic fungi simplifies complex materials for easy absorption.
True
Main difference of cell wall of plants and fungi
Plant: cellulose
Fungi: chitin
Fungi are subdivided on basis of?
- life cycles
- presence or structure of fruiting body
- arrangement and type of spores
Laymanβs description of fungi
- Mold
- Mildew
- Mushroom
- Bracket Fungi
- Puffballs
- Truffles
Similarities of Plants and Fungi
- Eukaryotic (numerous organelles)
- has cell walls
- anchored on soil or other subtrates
- reproduction can be asexual or sexual
- stationary
Difference of plants and fungi
Nucleus
Fungi: Multinucleated
Plant: Single nucleus
Difference of plants and fungi
Type of organism
Fungi: Heterotroph
Plant: Autotroph
Difference of plants and fungi
Main storage product
Fungi: Glycogen
Plant: Starch
Difference of plants and fungi
Cell Wall
Fungi: Chitin
Plants: Cellulose
Difference of plants and fungi
Reproduction
Fungi: not by seed
Plants: some by seed
Sterols in prokaryotes are absent except in?
Mycoplasmataceae
Part of the fungi that produces fruit (spores).
Fruiting bodies
This is a single vegetative cell
Yeast
Yeast appearance in culture
smooth, creamy colony without aerial hypha
Yeast is reproduced by?
budding or fission
Yeast reproduce by?
budding or fission
Yeast can be identified using?
Biochemical test and Molecular Diagnostic methods
Molds grow in multicellular filaments called?
hyphae
Example of Yeast
Candida spp.
These are made up of tubular branches, having multiple genetically identical nuclei, yet form a single organism, known as a colony.
Molds
Example for Molds
Trichophyton
Molds have an appearance of?
aerial hyphae
This has 2 forms, specifically yeast & mold, and are agents of systemic mycosis.
Dimorphic
Skeletal components of Cell Wall
- Chitin
- B Glucan
- Mannan
T/F: All components contributes to Cell Wall rigidity.
True
This refers to adjustment to shape of the habitat.
plasticity
The cell wall acts as:
- carrier of specific antigen characteristics
- filter controlling materials that enter the fungal protoplast
- site of various extracellular enzymes
- reservoir of carbohydrates
Cell Wall matrix consists of:
water-soluble polysaccharide (Glucan, Glycoprotein)
Miscellaneous CW component
Melanin
Melanin contributes to?
fungal virulence
Importance of Melanin in CW
- contributes to fungal virulence
- improves resistance to environmental damage
- important for invasion and dissemination
Melanin improves resistance to environmental damage such as?
extreme temperature, UV light, toxins
Membrane bound organelles in Fungi
- Nucleus
- Mitochondrion
- E.R.
- Golgi bodies
- Microbodies
These are plaque-like structure which plays the role of the centrosome by initiating organization of microtubule, during mitosis.
Spindle Polar Bodies (SPBs)
SPBs are embedded in the nuclear envelope for the entire cycle in?
budding yeast
These are vesicles associated with filamentous material.
Filasomes
These are found numerous at the tip of actively growing hypha
Filasomes
This refers to the body of the fungi.
Thallus
A web of filaments or hypha constitutes a?
Mycelium
This is a non-motile thallus constructed of apically elongated walled filaments.
Molds
These are branches or filamentous structures of fungi.
Hypha
These are filamentous tubular structures that grow by elongation (like thread) at the tip; or by branching that contain numerous nuclei distributed throughout.
Hypha
This refers to the entire, vegetative body of the hypha
Thallus
This is where the hyphal structures are interrupted at some points (at regular intervals) by partitions or cross walls
Septate
This is when portions of hyphae grow vigorously resulting in lack of regularly spaced septa.
Non septate
Other terms for Non septate
- Aseptate
- Coenocytic
Elements within the Hypha:
- Spitzenkorper
- Fungal cytoskeleton
This is the organizing center necessary for long range transport of vesicles, via cytoskeleton.
Spitzenkorper
This is described as nuggets of vesicles in the hyphal tip that is important in the growth process.
Spitzenkorper
These are important in filamentous fungi.
Fungal cytoskeleton
Importance of Fungal cytoskeleton
- important in regulation of fungal cell morphogenesis
- delivery of cell membrane and cell wall components to growing hyphal tip and to septum
This portion of the aerial hypha that bears the reproductive spores or conidia.
Reproductive hypha
Hypha that projects above the medium and produce reproductive spores.
Aerial hypha
These are portions of the hypha that penetrates the supporting medium and absorbs nutrient.
Vegetative Hypha
These are root like structures in fungi.
Rhizoids
Types of Hyphal Elements
Accdg to presence or absence of crosswall
- Septate
- Nonseptate
Types of Hyphal Elements
Accdg to presence of Pigmentation
- Hyaline (Moniliaceous)
- Phaeoid (Dematiaceous)
Non pigmented or lightly pigmented
Hyaline (Moniliaceous)
Darkly pigmented because of the presence of melanin in the cell wall
Phaeoid (Dematiaceous)
Hyphal shapes
- Antler (Favic Chandelier)
- Racquet
- Spiral
- Pectinate
- Rhizoids
- Nodular organ
Fungi with Spiral hyphae
Trichophyton mentagrophytes
Fungi with Pectinate body
Microsporum audouinii
Fungi with Antler hyphae
Trichophyton schoenleinii
Fungi with Nodular organ
Microsporum canis
Fungi with Racquet hyphae
Epidermophyton floccosum
Differences between Yeast and Molds
Habitat
Yeast: fruit and berries, stomach of mammals and on skin
Molds: damp, dark, steam-filled areas
Differences between Yeast and Molds
Cell
Yeast: Unicellular
Molds: Multicellular
Differences between Yeast and Molds
Shape
Yeast: Round or oval
Molds: Filamentous or threadlike
Differences between Yeast and Molds
Growth Appearance
Yeast: White, butterlike consistency
Molds: Fuzzy appearance, orange/green/black/brown
Differences between Yeast and Molds
Hyphae
Yeast: do not have true hyphae, have pseudo-hyphae instead
Molds: have hyphae
Differences between Yeast and Molds
Reproduction
Yeast: Asexual (budding), Blastospores
Molds: Sexual and asexual
Molds
Asexual spores
- Sporangiospores
- Conidia
Molds
Sexual spores
- Zygospores
- Ascospores
- Basidiospores
Differences between Yeast and Molds
Beneficial Use
Yeast: alcoholic beverages, baking, bioremediation, industrial ethanol production, probiotics
Molds: biodegradation, food production
Differences between Yeast and Molds
Health Hazard
Yeast: infection to immunocompromised patients
Molds: cause allergic reactions and respiratory problems
T/F: Yeast is not an opportunistic pathogen.
False
Having only one growth phase
Monomorphism
Examples of Monomorphism
Yeast, yeast-like organism
This is the ability of fungi to exist in two forms (yeast or mold) depending on growth condition
Dimorphism
Examples of Dimorphism
Blastomyces, Coccidioides
This is the ability to have both yeast and mold form in the same culture, regardless of temperature.
Polymorphism
Examples of Polymorphism
Exophiala spp.
Life cycle of Fungi
- Somatic phase
- Reproductive phase
This is considered as the feeding stage or energy absorption.
Somatic phase
This stage is for spore production.
Reproductive phase
T/F: Isolation of spores is done for identification of reproductive phase.
True
Fungi that reproduce sexually or asexually
Perfect Fungi
Fungi that reproduce by asexual means
Fungi Imperfecti
This involves the body of a fungal thallus, no production of seeds or spores by meiosis or syngamy.
Vegetative Reproduction
Asexual reproduction is through?
Mitosis
Types of Asexual reproduction
- Fragmentation
- Fission
- Budding
- Spore formation
Occurs when a fungal mycelium separates into pieces, with each component growing into a separate mycelium.
Fragmentation
This is the pinching off of an offspring from the parent cell.
Budding
T/F: In budding, the offspring cell is genetically identical to the parent.
True
Simple splitting of a cell into two daughter cells
Fission
This is a reproductive structure of fungi, and some other organisms, containing one or more cells.
Spore
This is a small unit of propagule capable of giving rise to a new individual.
Spore
These are readily dispersed and capable of germination when growth conditions are favorable.
Spore
Spores are more resistant to?
adverse condition
Spores can be derived from both?
asexual and sexual reproduction
These are produced after mitosis without involvement of meiosis, and are genetically identical.
Asexual spores
Simplest mechanism of spore formation
differentiation of preformed mycelium
Spores generated from asexual reproduction is termed as?
Thallospores
Major Types of Asexual Spores
- Sporangiospores
- Conidia
Spores that are produced in a sporangium (end of sporangiophores)
Sporangiospores
Enclosed, sac-like structure at the end of sporangiophores
Sporangium
Attachment point of sporangiospores
Columella
Spores produced at the tip or side of hyphae, on stalk, or conidiophores
Conidia
Special spore-producing structures
Conidiophores
Types of Conidia
- Arhtrospores
- Chlamydospores
- Blastospores
- Phialospores
- Macroconidia
- Microconidia
- Porospores
Process of conidium formation
Conidiogenesis
Conidia differentiate via expansion from the conidiogenous cell
Blastic
The cell wall of this cell is locally weakened and the developing conidium bulges out, and is delineated by a septum.
Blastic
Expansion of a cell that includes the complete cell wall (inner and outer) of the conidiogenous cell
Holoblastic
The cell wall of the conidiogenous cell is disrupted and conidium appears through an opening in the cell wall.
Enteroblastic
Preexisting cell differentiates into a conidium
Thallic
Involves entire cell wall (inner and outer) of hypha
Holothallic
Conidia produced in succession along entire length of hypha
Thallic-arthric
Conidiospores formed by buds coming off the parent cell
Blastoconidia
Conidia under Holoblastic
- Blastoconidia
- Poroconidia
Conidia under Enteroblastic
- Phialoconidia
- Anneloconidia
Conidia under Holothallic
- Chlamydospores
Example of Chlamydospores
Fusarium
Conidia under Thallic-Arthric
- Holothallic
- Holoarthric
- Enteroarthric
Examples of Holothallic
Epidermophyton
Examples of Holoarthric
Geotrichum
Examples of Enteroarthric
Geomyces
Conidium produced from annelide
Anelloconidium
Non motile sporangiospores
Aplanospores
Asexual conidium produced directly from the hyphae and released from this structure through fragmentation
Arthroconidia
Sexual spore formed on a basidium following karyogamy and meiosis
Basidiospore
Asexual spore produced blastically either singly or synchronously in chain from the parent cell
Blastoconidium
Swollen thick walled vesicles that do not reproduce
Chlamydospores
Asexual spores produced directly from the hyphae, have thickened walls and is larger than surrounding hyphal cells
Chlamydoconidium
Spore formed within a spherule by cleavage of the cytoplasm
Endospore
The larger of the two types of conidia produced through holothallic mode of condiogenesis
Macroconidium
Mycelia from which the reproductive structures form
Fertile hyphae
Conidium born from a phialide
Phialoconidia
Holoblastic conidium produced through pores in the cell wall of the conidiogenous cell or conidiophore
Poroconidium
Primary asexual reproductive structures formed in a sac called sporangium
Sporangiospore
Round thick walled spore formed in a zygosporangium by fusion of the tips of two compatible hypha
Zygospore
T/F: Meiosis in fungi occurs at a different point in the reproductive lifecycle than in other higher eukaryotes
True
3 stages of Sexual Reproduction
- Plasmogamy
- Karyogamy
- Meiosis
Union or fusion of haploid cells of compatible mating types
Plasmogamy
Fusion of the two haploid nuclei
Karyogamy
Newly produced diploid cell can undergo this to regenerate haploid cells, and often a response to nutrient limitation
Meiosis
Haploid nuclei do not fuse right away, coexist in the mycelium
Heterokaryotic Stage
Subphyla of Glomeromycota
- Mucoromycotina
- Entomophthoromycotina
- Kickxellomycotina
- Zoopagomycotina
Glomeromycota
Profuse to gray to white, aerial mycelia, hyaline sparsely septate hypha
Order Mucorales
Glomeromycota
Asexual and Sexual
A: Sporangiospores
S: Zygospores
Glomeromycota
Genera
- Actinomucor
- Apophysomyces
- Cokemyces
- Cunninghamella
- Lichteimia
- Mucor
- Rhizomucor
- Rhizopus
- Saksenaea
- Syncephalastrum
Ascomycota
Class
Ascomycetes
Ascomycota
Characteristics
Septate hyphae
Ascomycota
Reproduction
A: Conidia
S: Ascospores (sac or ascus in karyogamy)
Ascomycota
Examples
- Microsporum spp.
- Trichphyton spp.
- Scedosprium boydii
Basidiomycota
Characteristics
Sterile mold, hyphae has complex septa, Clamp connections (septation in vegetative hypha)
Basidiomycota
Reproduction
S: Four progeny basidiospores (supported by club shaped basidium)
Basidiomycota
Examples
- Filobasidiella neoformans (teleomorph)
- Cryptococcus neoformans (anamorph)
- Mushroom
Fungi Imperfecta is also known as?
Deuteromycetes
Fungi Imperfecta
Characteristics
artificial grouping
Fungi Imperfecta
Reproduction
S: not discovered
Anamorphic state: asexual conidia
Fungi Imperfecta
Examples
- Coccidiodes immitis
- Paraccocidiodes brasiliensis
- Candida albican
- Torulopsis
- Epidermophyton
Whole fungus, composed of sexual and asexual phase
Holomorph
Spores enclosed in an Ascus following karyogamy
Ascospores
Fruiting body of Ascospores
Ascocarp
Covering-like structure where Ascospores are enclosed
Ascus
Sexual life cycle of Ascomycetes
- Heterothallic (self-incompatible)
- Homothallic (self-compatible)
Exists in 2 mating types, occurs only between sexual structures of opposite mating type
Heterothallic
Every strain is able to complete sexual cycle without a mating partner
Homothallic
Major Types of the Multicellular ascomata
- Clesitothecium
- Perithecium
- Apothecium
- Pseudothecium
Examples of Cleisthomecium
Plectomycetes: Aspergillus nidulans
Examples of Perithecium
Pyrenomycetes: Neurospora crassa
Examples of Apothecium
Discomycetes: Peziza vesiculosa
Examples of Pseudothecium
Loculoascomycetes: Cochliobolus heterostrophus
Spores are formed inside a basidium (club shaped)
Basidiospores
Attachment point of Basidiospores
Sterigma
Thick walled spores formed by fusion of 2 hyphal strands (homothallic)
Zygospores
Fusion of cells from 2 separate non-identical hypha
Oospores