E2 LAB: PREPARATION OF CULTURE MEDIA

Question 1

Accurate diagnosis of fungal infections is critically dependent on?

Answer 1

effective isolation and identification of pathogenic fungi

Question 2

Inhibitory substances are added to?

Answer 2

nonsterile site specimens

Question 3

This is crucial for diagnosing suspected mycotic diseases.

Answer 3

Careful selection of culture medium

Question 4

Examples of Inhibitory Substances

Answer 4

• Chloramphenicol
• Cycloheximide

Question 5

Quality control includes?

Answer 5

monitoring expiration period of culture media

Question 6

under Dehydrated culture media

Answer 6

• Saboraud Dextrose Agar (SDA)
• Potato Dextrose Agar (PDA)
• Yeast Fermentation Broth
• Brain Heart Infusion

Question 7

Dehydrated culture media

Fungal cultivation and isolation of dermatophytes

Answer 7

Saboraud Dextrose Agar (SDA)

Question 8

Dehydrated culture media

General purpose basal medium, made from potato infusion and dextrose used to isolate yeasts and molds

Answer 8

Potato Dextrose Agar (PDA)

Question 9

Dehydrated culture media

Culture medium that is useful for yeast identification made from peptone and yeast extract to base

Answer 9

Yeast Fermentation Broth

Question 10

Dehydrated culture media

Nutrient-rich medium used to isolate fastidious fungi

Answer 10

Brain Heart Infusion (BHI)

Question 11

Other Media and Reagents

Answer 11

• Tween 80
• Distilled Water

Question 12

Equipment and Materials

Answer 12

• Beakers
• Erlenmeyer flasks
• Graduated cylinders
• Sterile Petri dishes
• Screw-capped tubes
• Analytical balance
• Spatula
• Stirring Rod
• Cotton
• Gauze

Question 13

Weighing for plated media uses?

Answer 13

Erlenmeyer flask

Question 14

Weighing for tubed media uses?

Answer 14

beaker

Question 15

Saboraud Dextrose Agar

Answer 15

65g, 1L

Question 16

Potato Dextrose Agar

Answer 16

40g, 1L

Question 17

Yeast Fermentation Broth

Answer 17

50g, 1L

Question 18

BHI Broth

Answer 18

37g, 1L

Question 19

BHI Agar

Answer 19

52g, 1L

Question 20

Tween 80

Answer 20

0.05mL (in a 125mL flask), 100mL

Question 21

This step is not necessary when preparing a liquid medium.

Answer 21

Heat to boil and dissolve medium

Question 22

After dissolving, plug the flask with?

Answer 22

gauze

Question 23

Sterilizing the flask with medium is done by?

Answer 23

autoclaving (15-20 minutes at 12C and 15-psi prssure)

Question 24

For tubed media, pour an amount of solution into?

Answer 24

screw-capped tubes

Question 25

Pour solution from flask into sterile Petri dishes using?

Answer 25

aseptic techniques

Question 26

For tubed medias, lay the tubes in what position?

Answer 26

in a slanted position

Question 27

This will give the fungus a relatively large surface to grow.

Answer 27

Sloping the agar

Question 28

For liquid media, leave the test tubes in what position?

Answer 28

in a vertical position

Question 29

Perform plating media inside a?

Answer 29

biosafety cabinet

Question 30

Store media at temperatures between?

Answer 30

1C and 6C