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MT6322 MYCOVIRO > E2 LAB: PREPARATION OF CULTURE MEDIA > Flashcards

E2 LAB: PREPARATION OF CULTURE MEDIA Flashcards

1
Q

Accurate diagnosis of fungal infections is critically dependent on?

A

effective isolation and identification of pathogenic fungi

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2
Q

Inhibitory substances are added to?

A

nonsterile site specimens

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3
Q

This is crucial for diagnosing suspected mycotic diseases.

A

Careful selection of culture medium

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4
Q

Examples of Inhibitory Substances

A
  • Chloramphenicol
  • Cycloheximide
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5
Q

Quality control includes?

A

monitoring expiration period of culture media

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6
Q

under Dehydrated culture media

A
  • Saboraud Dextrose Agar (SDA)
  • Potato Dextrose Agar (PDA)
  • Yeast Fermentation Broth
  • Brain Heart Infusion
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7
Q

Dehydrated culture media

Fungal cultivation and isolation of dermatophytes

A

Saboraud Dextrose Agar (SDA)

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8
Q

Dehydrated culture media

General purpose basal medium, made from potato infusion and dextrose used to isolate yeasts and molds

A

Potato Dextrose Agar (PDA)

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9
Q

Dehydrated culture media

Culture medium that is useful for yeast identification made from peptone and yeast extract to base

A

Yeast Fermentation Broth

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10
Q

Dehydrated culture media

Nutrient-rich medium used to isolate fastidious fungi

A

Brain Heart Infusion (BHI)

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11
Q

Other Media and Reagents

A
  • Tween 80
  • Distilled Water
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12
Q

Equipment and Materials

A
  • Beakers
  • Erlenmeyer flasks
  • Graduated cylinders
  • Sterile Petri dishes
  • Screw-capped tubes
  • Analytical balance
  • Spatula
  • Stirring Rod
  • Cotton
  • Gauze
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13
Q

Weighing for plated media uses?

A

Erlenmeyer flask

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14
Q

Weighing for tubed media uses?

A

beaker

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15
Q

Saboraud Dextrose Agar

A

65g, 1L

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16
Q

Potato Dextrose Agar

17
Q

Yeast Fermentation Broth

18
Q

BHI Broth

19
Q

BHI Agar

20
Q

Tween 80

A

0.05mL (in a 125mL flask), 100mL

21
Q

This step is not necessary when preparing a liquid medium.

A

Heat to boil and dissolve medium

22
Q

After dissolving, plug the flask with?

23
Q

Sterilizing the flask with medium is done by?

A

autoclaving (15-20 minutes at 12C and 15-psi prssure)

24
Q

For tubed media, pour an amount of solution into?

A

screw-capped tubes

25
Q

Pour solution from flask into sterile Petri dishes using?

A

aseptic techniques

26
Q

For tubed medias, lay the tubes in what position?

A

in a slanted position

27
Q

This will give the fungus a relatively large surface to grow.

A

Sloping the agar

28
Q

For liquid media, leave the test tubes in what position?

A

in a vertical position

29
Q

Perform plating media inside a?

A

biosafety cabinet

30
Q

Store media at temperatures between?

MT6322 MYCOVIRO (10 decks)

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