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MT6319 MOLBIO > U1 LAB: LAB SAFETY AND PIPETTING TECHNIQUES > Flashcards

U1 LAB: LAB SAFETY AND PIPETTING TECHNIQUES Flashcards

1
Q

Field involved in the examination of the origins of disease at the molecular level, primarily by studying nucleic acids

A

Diagnostic Molecular Biology

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2
Q

Hands on skills

A
  • Accuracy and precision
  • Specimen collection, handling, storage and disposal
  • Specimen processing
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3
Q

Micropipetting is for?

A

accuracy and precision

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4
Q

Specimen processing includes:

A

nucleic acid, protein extraction and detection

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5
Q

Knowledge required on the laboratory

A
  • Laboratory mathematics
  • Laboratory safety
  • Result analysis and interpretation
  • QA and QC
  • Social, ethical, legal issues
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6
Q

According to WHO, this refers to the containment principles, technologies, practices that are implemented to prevent unintentional exposure to pathogens and toxins, or their accidental release.

A

Biosafety

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7
Q

Agent of biological origin that can cause disease in humans.

A

Biological Hazard / Biohazard

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8
Q

Universal Precautions

A

applied to blood only

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9
Q

Agents are not known to cause a disease

A

BSL-1 (Yellow)

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10
Q

Agents are associated with human disease

A

BSL-2 (Light orange)

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11
Q

Agents are associated with human disease and are potentially transmitted as aerosols.

A

BSL-3 (Orange)

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12
Q

Agents of life threatening nature

A

BSL-4 (Red)

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13
Q

BSL-1 agents

A
  • E.coli JM109, DH5a
  • Saccharomyces cerevisiae
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14
Q

BSL-2 agents

A
  • Human blood or body fluids
  • E coli. 1057:H7
  • Clostridium botulinum
  • Retroviral vectors
  • Human cells in cell culture
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15
Q

BSL-3 agents

A
  • HIV
  • H1N1 Flu
  • Tuberculosis
  • Rabies
  • Yersinia pestis
  • West Nile Virus
  • SARS-Cov2
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16
Q

BSL-4 agents

A
  • Ebola
  • Small pox
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17
Q

2 laboratories in BSL-4

A

Suit and Cabinet Laboratory

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18
Q

PPE in BSL-4

A
  • Positive pressure suits
  • Double ended autoclave
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19
Q

BSC Class II, type A1

A
  • 70% airflow through HEPA cabinet
  • 30% back into the room
  • Plenum is under positive pressure
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20
Q

BSC Class II, type A2

A
  • Plenum is under negative pressure
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21
Q

BSC Class II, type B1

A
  • 30-40% HEPA cabinet
  • 60-70% HEPA outside
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22
Q

BSC Class II, Type B2

A
  • 100% of air is exhausted outside (No air recirculation)
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23
Q

BSC Class II, Type C1

A

either Type A or Type B

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24
Q

This is developed by OSHA for employees who may be exposed to hazardous chemicals in workplace.

A

Hazard Communication Standard

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25
Q

These are substances that affect cellular development of a fetus or embryo.

A

Teratogens

26
Q

These are cancer causing substances.

A

Carcinogens

27
Q

These are chemicals with flashpoint below 37.8C

A

Flammable

28
Q

These are chemicals with flashpoint above 37.8C

A

Combustible

29
Q

These chemicals are injurious to the skin or eyes by direct contact or to the tissue of the respiratory and GIT if inhaled or ingested.

A

Corrosive

30
Q

These are substances that spontaneously explode or ignite or that evolve heat or flammable or explosive gases.

A

Reactive

31
Q

Direct electrical hazard

A

death, shock, burns

32
Q

Indirect electrical hazard

A

fire, explosion

33
Q

This refers to destroying all microbial life including spores.

A

Sterilization

34
Q

This refers to destroying majority of microbial life; but not necessarily spores.

A

Disinfection

35
Q

These are chemical disinfectants which can be safely applied to the skin or mucous membrane and are used to prevent infection by inhibiting the growth of bacteria.

A

Antiseptics

36
Q

Physical Methods

Dry heat

A
  • Red heat
  • Flaming
  • Incineration
  • Hot air oven
37
Q

Physical Methods

Moist heat

A
  • Pasteurization
  • Boiling
  • Autoclave
38
Q

Physical Methods

Uses what filter paper?

A

nanopore

39
Q

Chemical methods

A
  • Bleach
  • Ethanol
  • Hydrogen peroxide
  • Ethylene oxide
  • Paraformaldehyde
40
Q

Holding period for Autoclave

A

121C at 15 pound pressure for 15 minutes

41
Q

Holding period for Hot Air Oven

A

160C for 1 hour

42
Q

T/F: Test tubes and flasks should be wrapped in paper before putting in oven.

A

True

43
Q

These are widely used to remove unwanted microorganisms from liquids.

A

Membrane filters

44
Q

Filters are made with what material, and what is the average pore diameter?

A

cellulose esters, 0.22mm

45
Q

Chemical Methods

What percentage of alcohol or bleach is recommended?

A

70% Alcohol, 10% Bleach

46
Q

This has a very high penetrative power that can be highly lethal to all cells.

A

Ultraviolet (UV)

47
Q

UV is used for?

A
  • Cleaning surfaces (inoculation hoods)
  • Laboratory benches
  • Laboratory apparatus
48
Q

Three conditions for a successful aseptic technique

A
  • Work area must be disinfected
  • Transfer instruments/glasswares must be sterilized
  • Work must be accomplished quickly and efficiently
49
Q

This container is to be lined with clear autoclave bags and should be decontaminated inside the biosafety cabinet and/or autoclaved.

A

Biohazard containers

50
Q

This container should used for contaminated culture tubes and glasswares that are washing and decontamination.

A

Decontamination bins

51
Q

The shaft of a pipette is also known as a?

A

tip holder

52
Q

The thumbwheel is also known as a?

A

Fine volume adjustment ring

53
Q

In this displacement, air is displaced by the piston.

A

Air displacement

54
Q

In this displacement, aspiration force remains constant, unaffected by physical properties of sample

A

Positive displacement

55
Q

T/F: In air displacement, the volume of air displaced is equivalent to volume of liquid aspirated.

A

True

56
Q

This is preferred when dispensing aqueous solutions, which may contain low detergents and protein concentrations.

A

Forward Pipetting

57
Q

This is performed when dispensing viscous or foaming liquids, or very minute amt of liquid.

A

Reverse Pipetting

58
Q

When should you pre-rinse a pipette?

A
  • Changing a tip
  • Increase a volume setting
59
Q

Wiping the pipette is only necessary when?

A

when pipetting viscous liquids

60
Q

Choice of tissue for wiping the pipette

A
  • Resistant
  • Lint free
  • Inert to acids and solvents
61
Q

Fluid depth penetration

A

only <3mm

MT6319 MOLBIO (6 decks)

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