U1: INTRO TO MOLBIO AND DIAGNOSTICS Flashcards
This refers to biological activity at the molecular level.
Molecular Biology
This is the field that studies composition, structure, interaction of cell molecules, which includes nucleic acids and proteins that carry out biological processes essential for cell function and mainteinance.
Biology
Why is molecular biology important?
Mixture of both biology and chemistry
The devlopment of molecular biology started with?
Genomics
DNA
detecting the genes
RNA
detecting the expression
Proteins
detecting if gene possesses a product
How many genes are there in the human body?
25,000
Molecular techniques:
- Polymerase Chain Reaction
- Molecular cloning
- Gel electrophoresis
- Macromolecule blotting and probing
- Microarrays
- Allele-specific oligonucleotide
Most common molecular technique
PCR
This blot detects DNA.
Southern
This blot detects RNA.
Northern
This blot detects proteins.
Western
The one who developed Southern Blot is?
Edwin Southern
This is not generally translated, but it is the post translation of proteins.
Eastern Blot
These are the 2 world’s best publications who published the initial sequence in 2001.
Nature and Science
Final draft of Human Genome Project was published on?
2003
This term is synonymous to selection of therapeutic regimens and tailor fit to individual patients.
Personalized Medicine
This refers to susceptibility test aiming at a low risk population.
Genetic Screening
This refers to analysis of DNA or biomarkers for evaluation of one or more genetic risk factors for a particular disease or disease group.
Genetic Testing
T/F: There are many factors that affect overall composition or expression.
True
