Types of Fixatives Flashcards
Made of only 1 component substance
Simple fixatives
Made of 2 or more fixatives
Compound fixatives
Permit the general microscopy study of tissue structure
Microanatomical fixatives
Microanatomical fixatives
10-10
H-F
Z-Z
B-B
Preserve specific parts and particular microscopic elements
Cytological fixatives
Cytological fixatives
NCH
Preserve nuclear structure; contain glacial acetic acid due to its affinity for nuclear chromatin; pH < 4.6
Nuclear fixative
Nuclear fixatives
BFNCH
Preserve cytoplasmic structures; do not contain glacial acetic acid; pH > 4.6
Cytoplasmic fixatives
Cytoplasmic fixatives
HORFF
Preserve chemical constituents of cells and tissues
Histochemical fixative
Histochemical fixatives
10F-ANA
Both nuclear and histochemical fixative
Newcomer’s fluid
Fixed as soon as after death
Autopsy materials
Fixed as soon as possible after removal
Surgical specimens
Amount of fixative for prolonged fixation (e.g. museum prep)
50-100x
Packed with cotton soaked in fixative
Hollow organs
Should not be used for glycogen containing tissues
Water (glycogen is soluble in water)