Transposons Flashcards

1
Q

When were transposons first discovered?

A

In 1940, when genetic elements from maize moved from site to site

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2
Q

What % of human genes are transposons?

A

50%

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3
Q

Why do transposons need transposase?

A

Bind at specific recombinase providing the ability for it to hop between locus’

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4
Q

What target bias to transposons show?

A

They tend to avoid highly expressed genes

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5
Q

How frequent does transposition occur?

A

every 100- 1 million cell divisions

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6
Q

Asides from tranposase genes what else must a transpon carry

A

Inverted repeat flanks

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7
Q

What is the cut and paste mechanism of jumping?

A

DNA splicing will cut out the target sequence, where the transposon will then insert itself

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8
Q

What are the IS elements of a transposon?

A

A tranposase with inverted flanking repeats

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9
Q

What are composite transposons

A

Contain a central non-tranpositional core, with an IS element on either side

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10
Q

Why can’t TN3 and TN7 land near eachother?

A

Due to them being the same type, Prevents them from inactivating the original

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11
Q

What can transposons be used for?

A

Can be used to produce the randomly marked areas of mutation

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12
Q

Why is TN5 used within the lab to map mutageneis of transposons?

A
  • High jump frequency
  • Little specificity
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13
Q

What are integrons?

A

Gene acquisition hotspots which allow for rapid evolution

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14
Q

What is phage MU?

A

A bacteriophage of E.coli

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15
Q
A
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