The cellular envelope Flashcards
Where is the bacterial cell envelope found?
Outermost coat of the bacteria
What are the main functions of the bacterial cell envelope?
- Cellular protection
- Transport of nutrients
- Cell metabolism
What is the difference between the structure of a gram positive or gram negative wall?
Gram +ve has a thick peptidoglycan layer
Gram -ve has an outer membrane
When gram staining what color are gram positive bacteria?
Blue/purple
What is the function of the cytoplasmic membrane?
To generate energy and actively transport nutrients
Where are the brauns lipoproteins found?
Within the outer membrane
Name all the components of all the outer membrane
- Lipid bilayer
- Porins
- Brauns lipoprotein
- Periplasm
- Peptidoglycan
Which part of the LPS is highly varied among species?
The O-antigen
Which part of the bacterial cell is susceptible to hydrolysis by lysosomes?
Peptidoglycan
What is bacterical cell fractionation?
Taking apart individual components of the cell
How do we isolate the surface appendages?
Via centrifuging and removing the whole cells
How do we isolate the periplasm?
Osmotic shock
What are crude fractions?
Brute force lysis of the cells
What are some examples of crude fraction techniques
French press, sonication, Centrifuging at high speeds
