Transplantation Immunogenetics Flashcards
Serological cross match prevents?
Hyper acute rejection but ab formation still leads to acute humoral rejection and chronic graft rejection
Functional genomics?
How genes,transcripts and proteins İnteract
Transcriptomics?
Identify and quantify all transcripts based on stage or tissue specific expression
Proteomics?
Identify production and turnover of proteins
Identify alterations
Immunomics
Identify genes involved in immune regulation
Pharmacogenomics
Interaction of drug with various biochemical pathways
What is HLA?
Set of genes on chromosome 6
Human leukocyte antigens
Control graft rejection
Extraordinary polymorphism
Ag presentation
Where does HLA found?
Chromosome 6 short arm
6p21,3
What are the HLA classes ?
HLA 1
HLA 2
HLA 3
HLA 1?
On all nucleated cells
Constitutive
Least density in— skeletal muscle, brain ,
Intermediate density in— kidney,heart,liver
Most density in— lymphocytes
B,C,E,A,G,F
HLA 2?
Limited expression— Monocytes, Macrophages, Dendritic, Kupffers cells, Renal mesangial cells
Inducible— endothelium, activated T cells
DP,DM,DQ,DR
HLA present ag to?
T cells
HLA 1 present ag to which receptor of T cells?
CD8+
HLA 2 presents ag to which receptor of T cells?
CD4
In which class of HLA virus is involved?
HLA 1
MHC?
Control immune response
Present peptides
Promiscuous
High gene density
T cell interaction— structural stability
Universe of Ag and parasites— variability and polymorphism
HLA-E?
Doesn’t present any ag
Binds to inhibitory receptors on NK cells
Act as an indicator of classical class 1 expression
Located at the interface between the innate and immune system
Modulator of NK and Cytotoxic T lymphocytes activation and inhibitory function.
HLA-G?
Not antigen presenting
Trophoblast,thymus,cornea
Limited distribution and expression
Variety of isoforms
Expressed as a dimmer on the cell membrane
Role in maintenance of pregnancy
Exert inhibitory effect on immune cells-important in tumor escape and protection of allege in fetus.
MHC class 1 chain related genes?
HLA-1 like mol.
Seven MIC genes within MHC
MIC-A and MIC-B codes for functional products
Limited tissue distribution
Highly polymorphic
HFE gene?
HLA-1 like mol.
Gene responsible for hemochromatosis
Non-immunological function
Proteosome subunit beta type 8?
Gene in HLA-2 region
Essential part of the normal metabolic turnover of a cell
TAP1 and TAP2 genes?
HLA-2 region gene
Involved in the transport and loading of peptides into the class 1 mol.
Tapasin?
HLA-2 gene
Facilitate peptide binding into the class 1 groove
HLA-DM/DO ?
HLA-2 genes
Ensure loading of exogenous proteins into class 2 groove.
Prevents endo peptide from binding through invariant chain.
