Transplantation Immunogenetics

Question 1

Serological cross match prevents?

Answer 1

Hyper acute rejection but ab formation still leads to acute humoral rejection and chronic graft rejection

Question 2

Functional genomics?

Answer 2

How genes,transcripts and proteins İnteract

Question 3

Transcriptomics?

Answer 3

Identify and quantify all transcripts based on stage or tissue specific expression

Question 4

Proteomics?

Answer 4

Identify production and turnover of proteins
Identify alterations

Question 5

Immunomics

Answer 5

Identify genes involved in immune regulation

Question 6

Pharmacogenomics

Answer 6

Interaction of drug with various biochemical pathways

Question 7

What is HLA?

Answer 7

Set of genes on chromosome 6
Human leukocyte antigens
Control graft rejection
Extraordinary polymorphism
Ag presentation

Question 8

Where does HLA found?

Answer 8

Chromosome 6 short arm
6p21,3

Question 9

What are the HLA classes ?

Answer 9

HLA 1
HLA 2
HLA 3

Question 10

HLA 1?

Answer 10

On all nucleated cells
Constitutive
Least density in— skeletal muscle, brain ,
Intermediate density in— kidney,heart,liver
Most density in— lymphocytes
B,C,E,A,G,F

Question 11

HLA 2?

Answer 11

Limited expression— Monocytes, Macrophages, Dendritic, Kupffers cells, Renal mesangial cells
Inducible— endothelium, activated T cells
DP,DM,DQ,DR

Question 12

HLA present ag to?

Answer 12

T cells

Question 13

HLA 1 present ag to which receptor of T cells?

Answer 13

CD8+

Question 14

HLA 2 presents ag to which receptor of T cells?

Answer 14

CD4

Question 15

In which class of HLA virus is involved?

Answer 15

HLA 1

Question 16

MHC?

Answer 16

Control immune response
Present peptides
Promiscuous
High gene density
T cell interaction— structural stability
Universe of Ag and parasites— variability and polymorphism

Question 17

HLA-E?

Answer 17

Doesn’t present any ag
Binds to inhibitory receptors on NK cells
Act as an indicator of classical class 1 expression
Located at the interface between the innate and immune system
Modulator of NK and Cytotoxic T lymphocytes activation and inhibitory function.

Question 18

HLA-G?

Answer 18

Not antigen presenting
Trophoblast,thymus,cornea
Limited distribution and expression
Variety of isoforms
Expressed as a dimmer on the cell membrane
Role in maintenance of pregnancy
Exert inhibitory effect on immune cells-important in tumor escape and protection of allege in fetus.

Question 19

MHC class 1 chain related genes?

Answer 19

HLA-1 like mol.
Seven MIC genes within MHC
MIC-A and MIC-B codes for functional products
Limited tissue distribution
Highly polymorphic

Question 20

HFE gene?

Answer 20

HLA-1 like mol.
Gene responsible for hemochromatosis
Non-immunological function

Question 21

Proteosome subunit beta type 8?

Answer 21

Gene in HLA-2 region
Essential part of the normal metabolic turnover of a cell

Question 22

TAP1 and TAP2 genes?

Answer 22

HLA-2 region gene
Involved in the transport and loading of peptides into the class 1 mol.

Question 23

Tapasin?

Answer 23

HLA-2 gene
Facilitate peptide binding into the class 1 groove

Question 24

HLA-DM/DO ?

Answer 24

HLA-2 genes
Ensure loading of exogenous proteins into class 2 groove.
Prevents endo peptide from binding through invariant chain.