Transplantation

Question 1

Hyperacute Rejection

Answer 1

From preexisting Abs against donor tissue, causes irreversible rejection within minutes

Question 2

Cross Match Test

Answer 2

Test serum of recipient w/ WBCs from donor to see immune difference (preferably w/ Bs and Ts separated to see MHC I or II). Also add complement

Question 3

Acute Rejection

Answer 3

Tcs to HLA and shit, occurs 100% of time without identical twins. Occurs several weeks after transplant and controlled w/ immunosuppression

Question 4

Mixed Lymphocyte Reaction

Answer 4

Add donor lymphocytes in blood (inactivated) w/ active recipient lymphocytes in blood and see if the latter replicate for a direct response to donor shit

Question 5

3 Tests for Immune Difference

Answer 5

Cross Match Test
Mixed Lymphocyte Rxn
Genotypic Analysis

Question 6

Direct vs. Indirect Allorecognition in Rejection

Answer 6

Direct is from donor APC leaving tissue and going to LN

Indirect is from host APC taking up Ag and then presenting

Question 7

2 Global Suppressors

Answer 7

Cytotoxic drugs like cyclophosphade which akylylates DNA to kill activately reproducing cells
Steroids which shut down an assload of functions