Transpiration Flashcards

1
Q

What is the definition of transpiration?

A

The loss of water from the leaves by evaporation from the mesophyll cells followed by diffusion of water vapour through air spaces and stomata.

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2
Q

What is the transpiration stream?

A

Water taken in through roots, transported around the plant through a system of tubes, and lost through leaves in transpiration.

Transpiration is necessary as it ensures water moves continually around the plant.

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3
Q

Why are there more stomata on the lower surface of the leaves of most plants than the upper?

A

The lower surface is shielded from heat and sun.

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4
Q

How do stomata work?

A

When there is little water in the plant the plant the guard cells become flaccid and remain closed.

When there is an abundance of water in the plant the guard cells become turgid, opening the stomatal pore.

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5
Q

What are the factors influencing the rate of transpiration?

A

Temperature- water evaporates faster at warmer temperatures

Wind speed- moves water vapour away from the leaf to keep a steep concentration gradient

Humidity- transpiration is reduced in humid conditions as there is a shallow concentration gradient

Surface area of leaf- more space for stomata therefore more transpiration

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6
Q

What plant vessels carry water?

A

Xylem

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7
Q

What are the adaptations for Xylem vessels?

A

Specialised cells for carrying water.
No top or base so cells form continuous hollow tube
Run from roots to stem and subdivide into veins in the leaves.

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8
Q

What is active transport?

A

Uptake of minerals from the soil by root hair cells against the concentration gradient- requires energy

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9
Q

What is passive transport.

A

Diffusion, such as osmosis

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10
Q

What is the definition of diffusion?

A

Random movement of molecules from an area of high concentration to an area of low concentration, down a concentration gradient.

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11
Q

What is the definition of Osmosis?

A

Net movement of water molecules down a concentration gradient from an area of greater water concentration to an area of lower water concentration though a partially permeable membrane.

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12
Q

What are the factors that affect the movement of substances across plasma membranes?

A

Size of particles
Concentration gradient

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13
Q

What happens to animal cells when placed in pure water?

A

Osmosis will occur as water goes down the concentration gradient into the cell, until it swells and bursts (cell lysis)

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14
Q

What happens to animal cells in concentrated solutions?

A

Water diffuses out of the cell and it shrivels up.

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15
Q

What do plant cells do when placed in pure water.

A

They swell up, but don’t burst. The cell wall resists the cytoplasm pushing against it. It becomes turgid.

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16
Q

What happens to plant cells when placed in a concentrated solution?

A

The cell loses water and becomes flaccid. It stops pushing against the cell wall, and may be torn from it, becoming plasmolysed

17
Q

How would you perform the Potato cylinder experiment?

A

Label 5 boiling tubes 1-5

Add varying concentrations of sucrose solution, from 20% to 0%, in each tube

Cut 5 pieces of potato using a cork borer (remove all skin)

Weigh each potato

Place each piece of potato into each tube

Leave the potatoes overnight

Remove each potato from its cylinder and pat dry

Reweigh the potato

Construct a table recording the initial and final mass of the potatoes in each solution and use it to calculate the change in mass and % change in mass

18
Q

How do you use a water uptake potometer

A

Fill the potometer, making sure there are no air bubbles

Take a section of your plant and cut the bottom cm of the stem

Insert the cut end into the potometer, using Vaseline to create an air tight seal.

Remove apparatus from the water and turn the valve to allow water to move through the capillary tube.

Apply allocated factors (temperature, wind, humidity, etc).

Record the distance moved by the bubble every few minutes

Record the results in a table.

19
Q

How would you use a weight potometer

A

Use 2 similar well watered plants.

Enclose the pot of both plants in a polythene bag (make sure it’s air tight) leaving the plant open.

Record the mass of both plants.

Place each plant on different sides of the room with different environmental factors (e.g, one next to a fan and the other in still air).

Record the mass of each plant at the end of the session.

Calculate the % change in each plant.

20
Q

How would you demonstrate osmosis using visking tubing

A

Fill the 250cm beaker 3/4 full of tap water.

Take a 20cm length of visking tubing and place this in the beaker of water for 2 minutes to soften.

Take the tubing out of the water and rub it using your finger and thumb.

Tie a tight knot close to one end of the tube.

Using a syringe add 10cm3 of 20% sucrose solution to the visking tubing.

Tie a knot at the other end of the tube.

Pat the surface of the tube dry.

Weigh the tube with a top pan balance.

Record the mass on your table.

Remove the sausage from the water after 10 minutes.

Reweigh the sausage and record new mass.

Repeat steps 10 and 11 for 20 and 30 minutes