Translation Termination in Prokaryotes Flashcards
1
Q
When does termination occur?
A
When a stop codon enters the A-site
2
Q
Why can’t EF-Tu bring anymore aminoacyl-tRNA to a stop codon?
A
There are no tRNA corresponding to stop codons.
3
Q
List the three release factors involved in prokaryotic transcription termination.
A
RF-1, RF-2, RF-3
4
Q
What does RF-1 do?
A
Recognizes UAA and UAG
5
Q
WHat does RF-2 do?
A
Recognizes UAA and UGA
6
Q
What does RF-3 do?
A
Stimulates RF-1 and RF-2 binding
7
Q
What is peptidyl-tRNA hydrolysis in prokaryotic translation termination?
A
When RF occupies the A-site, the 50S subunit breaks the bond between C-terminal end of polypeptide and the 3’ OH end of the tRNA by adding water.
8
Q
What happens to the ribosome after peptidyl-tRNA hydrolysis?
A
Its components dissociate.
