Translation Flashcards
1
Q
tRNA loading
A
- load with AA by tRNA synthetase
- attach covalently
2
Q
ribosome
A
- 30S controls mRNA decoding site
- 50S contains peptidyl transferase that helps form peptide bonds
3
Q
IF1
A
induces ribosome to dissociate
4
Q
IF3
A
binds to 30S submit forming IF3-30S-mRNA complex to prevent reassociation of ribosome
5
Q
IF2
A
- complexes with GTP and initiator tRNA-fmet
- directs binding of tRNA to 30S
6
Q
IF3 dissociates
A
- 50S subunit reassociates
- GTP hydrolyzes - GDP, IF3, and Pi leave
- fmet-tRNA on top of start codon in P site
7
Q
elongation
A
A site accepts next tRNA
8
Q
Ef-Tu
A
- brings next tRNA with use of GTP and moves to A site
- forms peptide bond
9
Q
23 subunit
A
- helps form peptide bond
10
Q
Ef-G
A
- helps transfer tRNA from A site to P site with help of GTP
- tRNA goes to E site and leaves
11
Q
Termination
A
- termination codon enters A site
- Ef-Tu can’t bind - translation stalls
12
Q
RF1/RF2
A
release polypeptide from tRNA in P site
13
Q
RF3
A
removes RF1/RF2
