Regulation DNA initiation Flashcards
1
Q
Regulating what
A
DnaA to # of A boxes
2
Q
What happens at origin
A
each chromosome has 1 origin of rep located near DnaA gene
3
Q
in fast growing cells
A
- origin gets replicated so now there are two
4
Q
newly synthesized DNA
A
- hemi-methylated on old (parent strand)
- contains A box
5
Q
SeqA
A
- binds to hemi-methylated strand and sequesters to cell membrane.
- DnaA has no access now
6
Q
dam methylase
A
will methylate the new strand of the newly synthesized DNA if not sequestered to SeqA
7
Q
DnaA
A
will bind to the fully synthesized strand
8
Q
what does that make sure of
A
- to make sure that the origins fire at the same time once per cell cycle.
- prevents buildup of DnaA until GATC sequences fully methylated
