Bacterial Growth and Nutrition Flashcards

1
Q

anabolism

A
  • construct molecules from smaller units

- primarily reductive

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2
Q

catabolism

A
  • breaks down molecules into smaller units that are either oxidized to release energy, or used in other anabolic reactions
  • primarily oxidative
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3
Q

Biomass

A
  • What bacterium is made of
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4
Q

What bacterium is made of

A
  • 55% protein
  • 24% DNA/RNA
  • 9% Lipid
  • 6% Carb
  • 3% other organics
  • 1% inorganics
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5
Q

Macroelements

A
  • Carbon
  • Hydrogen
  • Nitrogen
  • Oxygen
  • Phosphorus
  • Sulfur
  • Iron
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6
Q

Autotrophs

A
  • get energy from the sun

- reduced inorganic chemicals

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7
Q

Heterotrophs

A
  • steal energy in the form of reduced carbon
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8
Q

Chemotrophs

A
  • chemicals as energy sources
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9
Q

Chemoautotrophs

A
  • derive energy from chemical reactions
  • synthesize all necessary compounds from CO2
  • use inorganic energy sources
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10
Q

Chemoheterotrophs

A
  • unable to fix carbon to form their own carbon compounds
  • must ingest preformed organic molecules
  • still obtain energy from oxidation of inorganic molecules
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11
Q

Phototrophs

A
  • light as an energy source
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12
Q

photoheterotrophs

A
  • organic electron donors
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13
Q

photoautotrophs

A
  • inorganic electron donors
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14
Q

Reduced molecules have

A
  • more energy

- more electrons

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15
Q

E. Coli’s carbon/energy source

A
  1. carbohydrate
  2. amino acids
  3. lipid
    4 nucleotides
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16
Q

Carbon

A
  • heterotrophs use organics to supply carbon

- autotrophs can “fix” CO2 from air

17
Q

Hydrogen

A
  • carbon source typically supplies H (H2O in autotrophs)
18
Q

Oxygen

A
  • Carbon source also typically supples O
19
Q

Nitrogen

A
  • Amino acids, NH4+, NO3-, (also N2-fixation)
20
Q

Phosphorus

A
  • almost entirely from inorganic P (PO42-)
21
Q

Sulfur

A
  • S-amino acids (cysteine), sulfate (SO42-)
22
Q

Micronutrients

A
  • typically inorganic (transition metals)
  • typically required in microgram quantities
  • often present in laboratory water and glassware
  • serve structural/catalytic roles in specific enzymes
23
Q

Manganese

A
  • involved in phosphate transfer
24
Q

Molybdenum

A
  • involved in nitrogen transformations
25
Q

Bug “Growth Factors”

A
  • typically small organic compounds
    • amino acids (protein synthesis)
    • purines/pyrimidines (nucleic acid synthesis)
    • vitamins (enzyme cofactors)
    • others include heme, cholesterol, polyamides
  • required in varying concentrations
26
Q

defined media

A
  • exact chemical composition is known
27
Q

complex media

A
  • chemical composition is poorly defined