Translation Flashcards
Open reading frame
The codons of an mRNA that are read sequentially (5’ to 3’) to specify amino acids
Triplet code
Each mRNA codon consists of 3 nucleotides
Continuous
The mRNA is read 3 nucleotides at a time without skipping any
Nonoverlapping
The mRNA is read in successive groups of 3 nucleotides
Redundant/degenerate
More than 1 codon occurs for each amino acid (3rd nucleotide might be different)
Allows for protection against mutation
Unambiguous
1 codon specifies 1 amino acid
Start and stop signal
Call for beginning and end of an open reading frame
AUG= start (codes for met)
Stop= nonsense codon (doesn’t code for amino acid)
Codon bias
Tendency of an organism to use a certain codon for 1 amino acid more than other codons
Wobble
Base pairing between tRNA anticodon and mRNA codon doesn’t necessarily follow complementary base pairing rules at the 3rd nucleotide
Reason: energetic favorability
Peptide bond
Covalent bond between amino acids
Formation catalyzed by ribosomes
R groups
Variable region of amino acids
Determine how polypeptide will fold and thus function
Nonpolar hydrophobic amino acids
Val Ile Pro Phe Ala Leu Met Gly Trp
VIP PALM GT
Polar hydrophilic amino acids
Cys Thr Ser Tyr Asn Gln
Come Taste STAG
Positively charged polar amino acids
His Arg Lys
Shallow HAL is a positive movie
Negatively charged polar amino acids
Asp, Glu