Genetic Mapping Flashcards
Linkage
Genes on the same chromosome are inherited together
Linkage group
Set of linked genes
Gene distance and recombination rates
The further apart 2 genes are, the more likely that there will be recombination between them
If the progeny look like the parentals, one can conclude that…
There is no recombination
Definition of map units and how to calculate them
Map units=% recombination
Map units= (number of recombinants/total number of progeny)*100
Double crossover
Occurs when 2 chiasmata form and there is double exchange between 2 sister chromatids
Progeny in lowest abundance result from…
Double crossover
Progeny in highest abundance is…
Parental
How to tell which gene in a test cross is in the middle
DCO tells which gene is in middle: only one that isn’t switched (odd one out)
Calculating map units between 2 traits with double crossover
Map units= [(# SCO progeny for trait + # DCO progeny)/ total progeny] * 100
Interference
One chiasma inhibits formation of another nearby chiasma
Likelihood of restoring parental phenotype in double crossover
The farther apart 2 genes are, the greater the probability that undetected crossovers will occur
Roughly equal number of offspring in testcross
No linkage of genes
4 high numbers from 8 phenotypic classes in testcross
Odd trait out assorts independently and two similar traits are linked
2 parental (high #), 4 SCO (intermediate #), and 2 DCO (low #) in testcross
Odd trait out is in middle and all traits are linked