Translation 2 Flashcards
Your 23 female patient is suffering from strept throat your attending physician has sent over a throat culture to the lab. Result show this strain seems to be the super strain of the millennia. Therefore your upon consultation with infectious disease, there must be a cocktail of antibiotics given to work in several different mechanisms.
The first antibiotic give in Chlorophemical and the second, tetracycline. Explain the 2 mechanism of action in which they inhibit the growth of the superbug.
. Secondly which medication is through to have the first and second action.
Upon the follow up visit 2 weeks later the patient is still with symptoms. Upon Calling EVAC a central line in put in for another administration of the Erthryomyocin. Again explain the mechanism of action here.
Chloropheminicol- Inhibits the catalytic peptide transferase in the 23s of the 50 subunits. therefore inhibiting the amino acid peptide bond. Therefore hindering translation.
tetracycline- mechanism is in such where it prevents the 30’s ribosome bind to the mRNA Sequence.
The first action here to work is the Chloropheminacol as it prevents further replication of the bacteria and its proteins. The 2nd action here is to preventive function to prevent more bacterial protein from beginning translation.
Ethryomyocin- acts upon the channel within the ribosome. Preventing the peptide to leave the 50s subunit of the ribosome.
Which enzyme is responsible for the succession of the pre-imitation. and how many ATP are needed for this process?
Aminoacyl tRNA Synthetase.
2 ATP
Name one process in which THF is known for in the Translation of prokaryotes. It is used in many cancer drugs such as folic acid analogs. If this process is halted which substrate is accomliated? and how does this affect the translation process of prokaryotes.
The initiator tRNA goes under a modification in which THF is used to create a Formyl- Methionine which is used for the start codon. this allows for the AUG codon to be recognized with the aid of Shine Delgardo Sequence
While looking for the shine delgado sequence which of the following would look?
A. 3 hydrogen bonds B. 2 Hydrogen bonds C. U Rich D. A Rich E. T Rich
A. 3 hydrogen bonds
This is so because the shine delgado sequence is rich in purine rich
which of the following is known to enter into the p site of the 50s of the ribosome?
The initiator tRNA, after that every tRNA molecule that enters must enter through the A site.
Within Prokaryotes what causes the dissociation of the ribosome subunits from the mRNA, once completed.
Release factor and GTP
Name 2 differences between prokaryotes and Eukaryotes during translation
Eukaryotes there is no Fmet Trna
promoter sequence in Eukaryotes is Kozak sequence which contains the AUG condon where as in the prokaryotes the Intiation sequence is shine delgado and the AUG codon is separate
During Eukaryotic initiation there is the 40s ribosome in which binds to several initiation factors however we begin to see that there is a drop in GTP molecule and eIF2 binds with GTP.
What is the next step?
The initiation is step becomes regulated, as it can complete the initiation complex.
Complete initiation complex= 40s ribosome, eIF2 with GTP and met-tRNA with the eIF4 on the 5’ methyl cap
After the Initiaton complex completes scanning for the Kozak sequence which is the next process in which takes place in which order
- 60s subunits comes along
- GTP is hydrolyzed to GDP
- AUG is recognized
A. 3>2>1
B/. 2>3>1
C. 1>3>2
d. 2>1>3
A.
- AUG is recognized
- GTP is hydrolyzed to GDP
- 60s subunits comes along
Which causes a conformational change within the 80 subunit of the ribosome?
eEF1a-GTP
Upon traveling to europe your patient mentioned getting sick but paying much mind to it. Her and 3 children present to your office with cough, fever. With note the father has been in contact and seems to have immunity. Upon sending antibiotics 2 days later they present to the office with the worsening symptoms. Upon reconnecting a comprehensive history the mother mentions she doesn’t believe in vaccination as she wasn’t either. After alerting the CDC for a diphtheria toxin outbreak and in isolation appriopriate measured where taken. Explain the mechanism in which diptheria toxin works;
Diptheriera toxin is inactivates eEF2 in which is responsible for the being take the GTP to become hydrolyzed to move the uncharged tRNA molecule to the next site within the 80s ribosome Hence preventing translocation .
to Chloropheminacol inhibits the catayltic peptidyl transferase, which is synomous within Eukaryotes?
A. Cyclohexamide
B. Dipertheria
C. Ricin
D. Tetracycline
Cyclohexmide- inhibits the eukaryotic peptidyltransferase
While drinking organic castor bean oil to loose weight your nearest KMART shaman, mention to take it small amounts. It could somewhat toxic. Why would this be so, and where would it affect?
A. Inactivattion to 80s subunits.
B. Catalyzes depryrmidation and inactivates the 23s subunit of rRNA
CCatalyzes depurination and inactivates the 28s subunit of rRNA
D. Everything goes to shit
C.Catalyzes depurination and inactivates the 28s subunit of rRNA
