Transcription & Regulation of Gene Expression 2

Question 1

Desiniing a Cancer drug however the focus of your drug is the having the body create an autoimmune response to it own RNA. if all MRNA molecule where targeted for breakdown in the lysosome, instead of the Cytosol? Protein synthesis would halt. Which Molecule is most likely responsible?

A. A modification at the 5’ of the coding strand
B. HDAC
C. HAT
D. ERSS
E. Mannose 6 Phosphate
F. RAB- GTP
E. a modification at the 3’ of the coding

Answer 1

A. Modification at the 5’ of the coding strand. this is such because the 5’ methyl cap on the MRNA sequence causes the mRNA molecule to leave the Nucleus via the nuclear pore and taregt the cytosol.

5’ -3’ Coding
3’-5’ Template
5’Methyl Cap-3’Poly a tail MRNA

Question 2

The molecule tropomyosin has encoded by a gene in which codes for over 40 types of tropomyosin molecules. These are extermley specific to their location. However one gene, with the gene being monocistronic. Which molecule is most vital within this process?

A. Introns 
B. Exons 
C. Splisoesomes 
D. Helicase
E.

Answer 2

C. Spilceosomes

This is responsible for removing the introns, therefore leaving the exons. Therefore giving the Mature RNA transcript, once Alternative splicing can occur.

Question 3

If looking to cancer and looking at the regulatory function with gene expression, if targeting the silencer sequences, the which molecule would be most likely effected as they are bound here?

A. ENahncer
B. Repressor
C. Actovator
D. Promoter

Answer 3

B. Repressor protein as they bind to the silencer sequence which prevents the transcription of the gene

Question 4

DNA is very complex in which has many regulatory mechanism, one such is the regulation via regulatory proteins. This is accomplished by a structural motif, there exhibiting several interactions with the DNA double helix of that sequence. Where of the following amino acids would you primarily see in regard to this? Where?

A. Leucine - Minor Groove
B. Lysine- Major Groove
C. Asparagine- Major Groove
D. Histidine- Major Groove

Answer 4

C. Aspargine - Major Groove

This occurs as the regulatory binds to the major groove of DNA helix to prevent. This is explained as a structural motif in which are responsible for the binding of regulatory proteins to Specific DNA Sequences

Question 5

As seen with Lac Operon being turned on by the prescece of Lactose, within Eukaryotes there is a same process, however when looking to trans-acting factors in which respond to trasnscriptinf factors. Which of the following modifications enzyme would most likely be used here?

A. HDAC
B. HAT
C. Methylation

Answer 5

B. HAT is resposible for acetylation in which causes the DNA to be open up in the heterochrrome portion in which would allow for more transcription to occur.

Question 6

The pieces of introns that are removed during RNA Spilcing are used for which type of RNA? what is their function?

A. MIcroRNA- Chew up the noncoding strands
B. SNRNA- chew up coding strand
C. TRNA- Transfer of Amino acids
D. MRNA- Coding for the exons

Answer 6

A MicroRNA

This type of MicroRNA is used to chew up the exon along side RISC molecules.

Question 7

While translation is initiating which is first to come in contract with the Polymerase molecule?

A. CAAT Box
B. TATA Box
C. Promoter Sequence
D. Repressor Region

Answer 7

A. CAAT Box which is -70 upstream from the Start Region.