Transgenics

Question 1

What is transgenic insect?

Answer 1

Insect that has a novel gene inserted into its DNA

Question 2

What are the two types of transgenics

Answer 2

Stable and paratransgenesis

Question 3

Whats is required for a stable transgenesis

Answer 3

Effector genes, regulation, germline transformation system, selection marker, drivers & population structure

Question 4

What do refractory gene do?

Answer 4

Makes the mosquito unable to transmit the pathogen

Question 5

What is a conditional sterility or lethal gene?

Answer 5

A gene that activated caused deleterious fitness effects

Question 6

What is a candidate gene for analysis

Answer 6

to study the effects of gene overexpression, knock-out and mutations

Question 7

How are transposable elements used?

Answer 7

Through the use donor and helper plasmids. Inverted repeats are inserted into donor plasmid, while the transposase gene is inserted into the helper plasmid, are then mixed together, and microinjected into the embryo.

Question 8

When is the helper gene lost?

Answer 8

Meiosis

Question 9

How efficient is transformation?

Answer 9

about 5%

Question 10

How do you make transgenic fluorescent progeny?

Answer 10

Transgene for fluorescent most be in the germ line. Are then crossed with the wild type, and the progeny which are transgenic are selected using a marker

Question 11

What is needed to spread a gene through a population?

Answer 11

A driver for the specific gene