Transcription

Question 1

What enzyme synthesizes the mRNA

Answer 1

RNA polymerase

Question 2

What DNA strand does RNA polymerase attach to

Answer 2

The template strand (3’ to 5’ strand)

Question 3

Direction mRNA is synthesized

Answer 3

5’ to 3’

Question 4

What is a promoter region

Answer 4

A special nucleotide sequence that lies just before a gene

Question 5

Where does transcription happen

Answer 5

Nucleus

Question 6

Transcription prokaryote vs eukaryote

Answer 6

Prokaryote
- Happens throughout cell
- Single type of RNA polymerase transcribes all genes
- Bases added quickly
- Less complex promoters
- Protein binds to the mRNA and cleaves or mRNA binds with itself during termination
- No introns
- mRNA ready to be translated

Eukaryote
- In nucleus
- Different RNA polymerases used
- Bases added slowly
- Promoters are immediately upstream of protein-coding genes and more complex
- Nuclear proteins bind to poly uracil site for termination
- Have introns and exons
- results in pre-mRNA which must be modified