topic 2 - organisation Flashcards
principles of organisation order them from smallest to largest
cell tissue organs organ system organism
cell
is the smallest structural and functional unit of an organism
tissue
a group of similar cells with similar structure and function
organ
a group of tissues working together to perform a specific function
organ system
a group of organs working together to perform a specific function
organism
a group of organ systems working together
define digestion
digestion is where large insoluble molecules are broken down into smaller soluble ones that can be absorbed into the bloodstream.
explain the digestion system
first food is chewed up in the mouth. enzymes in the saliva begin to digest the starch into smaller sugar molecules
the food then passes down the oesophagus and into the stomach. in the stomach, enzymes begin the digestion of proteins
the stomach produces hydrochloric acid which helps the enzymes digest proteins
stomach
produces hydrochloric acid
produces hydrochloric acid to kill bacteria
provide optimum ph for the enzymes to digest proteins
liver produces
bile which is stored in the gall bladder which helps with digestion of lipids
the large intestine
absorbs water from undigested food to produce faeces
small intestine
is where soluble molecules are absorbed into the bloodstream
glads - pancreas and salivary glands
produces digestive juice containing enzymes to break down the food
enzymes
are biological catalyst that speed the chemical reaction without being used up
define protein
are long chains of amino acids
what are enzymes made up of
enzymes are large proteins molecules
what are the lock and key? hypothesis
where the substrate has to have a complementary shape to fit the active site
all the key points of enzymes
enzymes are made of proteins and are biological catalyst that speed up the chemical reaction without being used up. the shape of the active site of enzymes is specific for each substrate.
what breaks down proteins
protease
what do protease convert proteins into
amino acid which is absorbed into the bloodstream
the stages of protease
proteins are broken down by enzymes called protease. when we digest proteins the protease enzymes convert into individual amino acid which is absorbed into the bloodstream. protease is made in the stomach, small intestine and pancreas
where are protease broken down into
stomach
small intestine
pancreas
what do carbohydrates break down into
carbohydrates
stages of carbohydrase
carbohydrates are broken down by enzymes called carbohydrase
carbohydrase convert carbohydrates into simple sugar
amylase is an example of carbohydrates it breaks down starch
amylase is made in 3 places - pancreas , salivary glands and the small intestine
carbohydrase convert carbohydrates into
into simple sugar
an example of carbohydrates
amylase
amylase breaks down into
starch
amylase is made in …
small intestine
salivary gland
pancreas
lipase convert lipids into
fatty acids and glycerol
lipase are made in
pancreas and small intestine
Soluble glucose, amino acids, fatty acids and glycerol pass into
into the bloodstream to be carried to all
the cells around the body.
They are used to build new carbohydrates, lipids and proteins, with
some glucose being
used in respiration.
where is the bile made and stored and released
bile made in the liver stored in the gall bladder
released in the small intestine
bile has 2 roles ;
-It is alkaline to neutralise the hydrochloric acid which comes from the stomach
emulsifies fats so breaks down large drops of fat into smaller ones
an example where bile emulsifies fats
bile converts large lipids droplets into smaller droplets
bile emulsifies the lipid what does this do to the surface area
increases the surface area so increase the rate of fat breakdown by lipase
bile is also an alkaline what does this do
neutralises stomach acid, creating an alkaline condition in the small intestine
enzymes require optimum
PH and temperature
the optimum temperature
the optimum temperature is around 37 degree
as the temperature increases the rate of reaction also increases this is because there is an increase collision between the substrate and enzymes
but if the temperature is too high
the enzymes will denature this is because at high temperature the enzymes vibrate and the shape of the active site changes
the optimum PH
The optimum pH for most enzymes is 7 and some produced in acidic conditions eg stomach
if the PH is too high or too low the active site denatures This will change the shape of the active site, so the substrate can no longer fit in
the forces that hold the amino acid chains that
make up the protein will be affected
Required Practical 5: Effect of pH on Amylase - method
- take 3 test tube and add starch solution, analyse solution and a buffer solution to each test tube
- place them in a water bath at 25c for a few minutes
- put a drop of iodine solution in each well of a spotting tile
- now combine the 3 solutions into one test tube and mix with a stirring rod
- after a 30-second transfer one drop of the combined solution to a well in the spotting tile that contains iodine
- the iodine should be blue-black meaning that starch is present
- repeat this process at fixed 30s interval until the iodine solution remains orange-brown
- when the iodine remains orange this tell us that starch is no longer present
- Calculate the rate of reaction using 1 / time taken for solution to remain brown
- Repeat at different pH values using different buffer solutions
Which enzyme catalyses the breakdown of starch in
the human digestive system?
Amylase - catalyses the breakdown of starch to
maltose
Why must samples be left in the water
bath before solutions are mixed?
To allow the temperature to equilibrate
What is a buffer solution?
A solution which can resist changes in pH
In experiments where you are measuring how much of a product forms over time or how much of a
a reactant is used up, you should calculate the rate using the equation:
rate = change/time
problems with this experiment
we are only taking samples every 30s so we only have an approximate time for reaction to complete
solution - shorten the time interval eg 10s
iodine takes time to turn blue/black - the colour change trends to be gradual
solution - several people to look at the spotting tile
Required Practical 4: Food Tests - Describe how you would prepare a sample of
food to be tested
first, prepare the food sample
grind up the food sample using a pestle and mortar
transfer the ground up food to a beaker and add some distilled water and stir it with a glass rod
filter the solution using a funnel lined with filter paper to get rid of the solid bits of food
Describe how you would test for the presence
of starch in a sample
use iodine solution to test for starch. make the food sample and transfer 5cm of your sample into a test tube add few droplets of iodine solution and if starch is present the iodine solution will turn from browny-orange to black-blue
Iodine test for
starch
what colour does iodine solution change into
orange to blue/black
Describe how you would test for the presence of
reducing sugar in a sample
place 2cm^3 of food solution into a test tube
add few drops of benedicts solution into the test tube
place the test tube into a water bath filled in hot water and leave this for 5 minutes
If reducing sugar is present, a brick red
precipitate forms. If not, the solution remains blue
the benedicts solution only works for certain sugars
reducing sugars eg glucose
Benedict’s test for
reducing sugar
Benedict’s test changes to
blue to brick red
but depending how much sugar there is in the food it can turn
green-small amount of sugar
yellow-more sugar present