Topic 2- Organisation Flashcards
What are large multicellular organisms made up of?
organ systems
what is the process where cells become specialised for a particular job called?
differentiation
when does differentiation occur?
during the development of multicellular organism
what do specialised cells form?
tissues
what is a tissue?
a group of similar cells the work together to carry out a particular function
Name three examples of tissues
Muscular tissue, Glandular tissue and Epithelial tissue.
What is the function of muscular tissue?
to contract to move whatever its attached to.
what is the function of Glandular tissue?
to make and secrete chemicals like enzymes and hormones.
what is the function of epithelial tissue?
to cover parts of the body.
give an example of parts of the body epithelial tissues cover
inside of the gut
what are tissues organised into?
organs
What are organs?
a group of tissues that work together to perform a certain function.
what three tissues is the stomach made up of?
Muscular tissue, Glandular tissue and epithelial tissue.
What is the function of Muscular tissue in the stomach?
moves the stomach wall to churn up food.
what is the function of glandular tissue in the stomach?
makes digestive juices to digest food.
what is the function of epithelial tissue in the stomach?
covers the outside and inside of the stomach.
How big is an epithelial cell?
less the 0.1 mm
How big is the stomach?
about ten cm, over 1000 times longer than an epithelial cell.
What are organs organised into?
organ systems
What is an organ system?
a group of organs working together to perform a particular function.
What does the digestive system do?
breaks down and absorbs food
what is the digestive system made up of?
glands , The stomach and small intestine, the liver, the small intestine and the large intestine.
What is a role of the glands?
e.g the pancreas and salivary glands which produce digestive juices.
what is the role of the stomach and small intestine?
they digest food
what is the role of the liver?
produce bile.
What is the role of the large intestine?
absorbs water from undigested food leaving faeces
What do organ systems that work together form?
organisms
What are enzymes?
biological catalysts produced by living things
why do chemical reactions in the body need to be carefully controlled?
to get the right amount of a substance.
How can you increase rate of reactions in the body?
raising the temperature.
Whats the disadvantages of raising the temperature to get a quicker ROR?
you would speed up the useful reactions but also the unwanted reactions too. also there a limi to how far you can raise body temperature before cells start to become damaged.
What do enzymes reduce the need of?
high temperatures. We only have enzymes to increase rates of useful reactions in the body.
What are catalysts?
a substance that increases the speed of a reaction with out being changed or used up in the reaction?
What are enzymes made up of?
large proteins in unique shapes.
What are proteins made up of?
long chains of amino acids.
Why do enzymes have special shapes?
so they can catalyse reactions.
What do chemical reactions usually involve?
things being split apart or joined together
What is the active site on a enzyme?
A unique shape that the substrate fits into.
How many reactions can enzymes catalyse?
usually one specific reaction.
why do enzymes only catalyse one reaction?
because the substrate has to match the enzymes active site for the reaction to be catalysed.
What diagram shows how enzymes work?
‘lock and key’ model.
How does the lock and key model differ from the actual process?
because the model doesn’t show ‘induced fit’ . (the active site actually changes shape slightly as the substrate binds to it for a tighter fit.)
What do enzymes need to work efficiently?
the right temperature and pH.
What does the temperature effect enzyme?
The higher the temperature the ROR increases. All enzymes have optimum temperatures they work best at.
What happens if the enzyme gets too hot?
bonds holding the enzyme together break, this changes the shame of the active site so substrates won’t fit anymore. the enzyme is denatured.
How does pH affect enzymes?
if the pH is too low or high the pH will interfere with the bonds holding the enzyme together. The shape of the active site changes and the enzyme is denatured.
Whats the most common optimum pH for an enzyme?
pH 7 (neutral), but this isn’t always the case.
give an example of an enzyme that doesn’t have an optimum pH of 7.
Pepsin - breaks down proteins in the stomach. It works best at pH 2. this means its more suited for the acidic conditions.
How do you carry out a practical that investigates the effect of pH on enzyme activity?
1- drop a spot of iodine solution into every well of a spotting tile.
2-place a bunsen burner on a heatproof mat, place a tripod and gauze onto of the bunsen burner. put a beaker of water onto of tripod and heat the water 35 degrees.
3- use syringe to add 1cmcubed of amylase solution and buffer solution (pH 5) to a boiling tube. using testable holders, put the tube into the beaker . and wait for five minutes.
4- use a different syringe to add 5cmcubed of a starch solution to the boiling tube.
5- immediately mix the contents and start stop clock
6- use a continuous sampling to record how long it takes the amylase to break down the starch. To do this use a dropping pipette to take a fresh sample from the boiling tube every 30 seconds and drop into a well. When the iodine remains browny orange starch is no longer present.
7- repeat the experiment with different buffer solutions of different pH values.
8-contol any variables each time, e.g concentration and volume of amylase.
how do you calculate rate of reaction?
rate = 1000/time or change in product formed/time
After enzymes breakdown big molecules such as starch, proteins and fats what happens?
the new smaller molecules such as amino acids, glycerol and fatty acids are soluble and can pass easy through the walls of the digestive system. They can then be absorbed into the bloodstream.
what converts carbohydrates into simple sugars?
carbohydrases.
give an example of a carbohydrase.
amylase (enzyme)
give an example of a carbohydrate.
starch.
name three places amylase is made.
salivary glands, the pancreas, the small intestine.
give an example of sugars.
maltose or dextrins
what converts proteins into amino acids.
proteases
where are proteases made?
the stomach, the pancreas and the small intestine.
what is protease called when its in the stomach?
pepsin
what converts lipids into glycerol and fatty acids?
lipase
what are lipids?
fats and oils.
what two places makes lipase?
the pancreas and the small intestine.
What is the purpose of bile?
neutralises the stomach acid and emulsifies fats.
Where is bile produced?
in the liver
where is bile stored?
Gall bladder
What organ is bile releases into?
The small intestine.
What is an issue with hydrochloric acid in the stomach?
makes the pH in the stomach acidic for enzymes in the small intestine to work properly.
How does bile help enzymes in the small intestine to work properly?
Bile is alkaline so it neutralises the stomach acid and makes conditions alkaline.
What conditions do enzymes work best at?
alkaline conditions
What does emulsifying fats mean?
Breaking down the fats into smaller droplets.
Why are fats emulsified?
it gives fats a greater surface area of fat for lipase to work on, digestion is faster.
What is made in the salivary glands?
amylase enzyme in the saliva.
What is the Gullet?
Oesophagus.
What enzyme does the stomach produce?
protease (pepsin)
Why does the stomach make hydrochloric acid?
To kill bacteria,
To give the right pH for pepsin to work (pH 2 - acidic)
What pH does the enzyme pepsin work best at?
pH 2 - acidic
What are the muscular walls of the stomach used for?
To pummel food.
What is produced in the liver?
Bile.
What is the purpose of the gall bladder?
To store bile and release it into the small intestine.
What does the pancreas produce?
Protease, lipase and amylase.
Where does the pancreas release enzymes to?
The small intestine.
What happens at the large intestine?
excess water is absorbed from food.
What does the small intestine produce?
amylase, lipase and protease enzymes to complete digestion.
What happens at the small enzyme?
digested food is absorbed out of the digestive system and into the blood.
What is the Rectum?
Where faeces ( indigestible food ) are stored.
How do you prepare food for food testes?
1- break up desired food using a pastel and mortar.
2- transfer food to breaker and add distilled water.
3- still mixture with a glass rod to dissolve some of food.
4- filter solution using a funnel lined with filter paper.
What is Benedicts solution used to test for?
Sugars
What time of sugar does Benedict’s solution test for?
Reducing sugars.
How do you carry out a food test with benedict’s solution?
1- Transfer 5cmcubed of a food sample to a test tube.
2-prepare a water bath set to 75 degrees.
3- add benedict’s solution to test tube ( ten drops) using a pipette.
4-Using a test tube holder place test tube in the water bath for five minutes, make sure the tube is pointing away from you.
What colour will Benedict’s solution change to?
It will turn from a blue colour to either, green, yellow or brick red depending on the amount of sugar in it.
What does iodine test for?
Starch
How do you carry out a food test with iodine?
1- add 5cmcubed of a food sample to a test tube.
2-add a few drops of iodine to the test tube and gently shake to mix the contents.
What colour will iodine go if starch is present?
It will go from browny - orange to black or black - blue
How do you test for lipids?
Sudan III solution
How do you carry out a food test using Sudan III solution?
1- add 5cmcubed of unfiltered food sample to a test tube
2- add three drops of the solution to test tube using a pipette and gently shake.
2-Sudan III solution stains lipids
How does Sudan III show that lipids are present?
The mixture will separate out into two layers, The top layer will be bright red.
Where are your lungs?
In the Thorax.
What is the Thorax?
The top part of your body.
What separates the thorax and the bottom part of your body?
The diaphragm
What are lungs protected by?
The rib cage.
What are lungs surrounded by?
Plueral membranes.