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KSUCPM CELL AND TISSUE > TISSUE STAINING I JBLE > Flashcards

TISSUE STAINING I JBLE Flashcards

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1
Q

what is cytology? histology?

A

the examination of cells

the examination of tissues

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2
Q

what are the different types of microscopy?

A

visible light microscopy

electron microscopy, two types (SEM and TEM)

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3
Q

what is the resolution of the visible light microscopy?

A

0.2 um (micrometers)

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4
Q

what are the magnifications of both TEM and SEM?

A

TEM 300,000 times

SEM 150,000 times

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5
Q

which of the electron microscopy techniques requires no sectioning or embedding?

A

SEM

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6
Q

why are copper grids important as mounts for electron microscopy?

A

they allow for the passage of electrons so we can see high resolution images at high magnification levels

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7
Q

what are the stages in the preparation of tissues?

A 
fixing
embedding
sectioning
mounting
staining
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8
Q

LM fixing?

A

formalin, formaldehyde solns (Zenkers, Helly’s Bouin)

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9
Q

EM fixing?

A

glutaraldehyde

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10
Q

LM embedding?

A

paraffin

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11
Q

EM embedding?

A

plastic

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12
Q

LM sectioning?

A

microtome

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13
Q

EM sectioning?

A

ultramicrotome

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14
Q

EM staining?

A

heavy metals, platinum, gold, osmium, lead

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15
Q

stages of tissue prep for SEM?

A 
fixative
no embed
no section
mounting
staining
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16
Q

how many types of objective lens are there for the light microscope?

A 
4 types
5x low
10x medium 
40x high
100x and 1000x oil immersion
17
Q

what is the benefit of frozen sections?

A

faster for examination when time is an issue

18
Q

what are the types of dyes used in staining? examples?

A

vital dyes (trypan blue/red, tritium, India ink) and supra vital dyes (janus green)

19
Q

this type of living tissue dye used in living host? used on living cultured tissues?

A

vital dye

supravital dye

20
Q

what are the types of histological or general or empirical stains? what are some examples?

A

acid stains, base stains, counter stains, trichrome

H&E
Mallory, Gomori, Masson, Wright, Van Glesson

21
Q

what are the types of histochemical stains? examples?

A

acid-base, metachromasia(structures stain a different color) vs orthochromatic(predicted stain), immunocytochemical

Antibody Proteins
Eosin-Methylene Blue
Orcein
Periodic Acid-Schiff Rxn
Resorcin Fuchsin
Silver
Sudan Dyes
Verhoeff's

KSUCPM CELL AND TISSUE (14 decks)

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