TISSUE STAINING I JBLE Flashcards
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what is cytology? histology?
the examination of cells
the examination of tissues
what are the different types of microscopy?
visible light microscopy
electron microscopy, two types (SEM and TEM)
what is the resolution of the visible light microscopy?
0.2 um (micrometers)
what are the magnifications of both TEM and SEM?
TEM 300,000 times
SEM 150,000 times
which of the electron microscopy techniques requires no sectioning or embedding?
SEM
why are copper grids important as mounts for electron microscopy?
they allow for the passage of electrons so we can see high resolution images at high magnification levels
what are the stages in the preparation of tissues?
fixing embedding sectioning mounting staining
LM fixing?
formalin, formaldehyde solns (Zenkers, Helly’s Bouin)
EM fixing?
glutaraldehyde
LM embedding?
paraffin
EM embedding?
plastic
LM sectioning?
microtome
EM sectioning?
ultramicrotome
EM staining?
heavy metals, platinum, gold, osmium, lead
stages of tissue prep for SEM?
fixative no embed no section mounting staining
how many types of objective lens are there for the light microscope?
4 types 5x low 10x medium 40x high 100x and 1000x oil immersion
what is the benefit of frozen sections?
faster for examination when time is an issue
what are the types of dyes used in staining? examples?
vital dyes (trypan blue/red, tritium, India ink) and supra vital dyes (janus green)
this type of living tissue dye used in living host? used on living cultured tissues?
vital dye
supravital dye
what are the types of histological or general or empirical stains? what are some examples?
acid stains, base stains, counter stains, trichrome
H&E
Mallory, Gomori, Masson, Wright, Van Glesson
what are the types of histochemical stains? examples?
acid-base, metachromasia(structures stain a different color) vs orthochromatic(predicted stain), immunocytochemical
Antibody Proteins Eosin-Methylene Blue Orcein Periodic Acid-Schiff Rxn Resorcin Fuchsin Silver Sudan Dyes Verhoeff's
