TISSUE PROCESSING & AUTOTECHNICON Flashcards
A branch of pathology which deals with the study of disease in a tissue section
Histopathology
What should be included in the identification of specimen preparation?
Name
Birthdate
Hospital # of the patient
Specimen source/site
What is the measurement for fine trimming in section-cutting?
4-6 micrometer
What is the temperature range of the autotechnicon
37 to 45 degrees Celsius
Function of the transfer arm
Moves the tissues from one station to another
Facilitates movement and exchange of processing reagents within the processing chamber
Fluid circulation system
How many wax changes is required to remove the clearing agents
2 to 3
Ensures thorough and uniform mixing of the specimens with the processing reagents
Agitation mechanism
Enumerate the steps of tissue processing
Fixation
Dehydration
Clearing
Impregnation/Infiltration
Embedding
Trimming
Section-cutting
Staining
Mounting
Labelling
What is the measurement range of a whole mount
0.2-0.5 mm
Made from blood, bone marrow or any fluid such as pleural or ascitic fluid
Smears
Most recommended routine dehydration
Ethyl alcohol
Process whereby alcohol or a dehydrating agent is removed from the tissue and replaced with a substance that will dissolve the wax with which the tissue is to be impregnated
Clearing
The process of removing the clearing agent from the tissue and replaced by a medium that will completely fill all the tissue cavities, giving the specimen a firm consistency
Infiltration
The last step in tissue processing that results in permanent histological preparation suitable for microscopy after ______ and ______
Mounting
Adhesions of the sections on to the slide; appropriate staining of the tissue
The autotechnicon passes tissues through its logical sequence of solutions by means of a carriage raised by a drive mechanism which, in turn, is operated by a ______
Clock-controlled timing disc
Gentle washing and minimal thickness of cell layers will prevent the cells from ______ during staining
Detaching
The autotechnicon have ____ individual processing steps with ____ one liter capacity glass beakers and ____ thermostatically controlled wax baths.
12; 10; 2
It is equipped with automatic dispensing mechanisms to ensure proper and consistent reagent delivery to the specimens
Reagent containers and dispensers
In general, needle biopsies and bloody specimens should be incubated _____, whereas fatty specimens can be processed for _____
Conservatively; longer than average
