FIXATION

Question 1

Specimen used in fixation of tissue

Answer 1

Fresh tissue

Question 2

Duration of fixation

Answer 2

2-6 hours

Question 3

Hypertonic solutions give rise to ______
Hypotonic and Isotonic solutions give rise to ______

Answer 3

Cell shrinkage
Swollen cells and poor fixation

Question 4

(2) aims of fixation

Answer 4

1. To preserve the morphologic and chemical integrity of cell in as life-like manner as possible
2. To harden and protect the tissue from the trauma of further handling

Question 5

What are the tissue cellular processes prevented by fixation?

Answer 5

Degeneration — Decomposition — Putrefaction — Distortion

Question 6

Recommended for the demonstration of chromatin, mitochondria, mitotic figures, Golgi bodies, RBC and colloid-containing tissues

Answer 6

Regard’s fluid

Question 7

Excellent for fixing dry and wet smears, blood smears, and bone marrow tissues

Answer 7

Methyl alcohol

Question 8

Used for fixing touch preparations

Answer 8

Isopropyl alcohol

Question 9

Formalin pigments are eliminated or reduced by fixation in _____ or ______

Answer 9

Phenol-formalin; neutral buffered formalin