DEHYDRATION, CLEARING & INFILTRATION Flashcards
For urgent biopsies, dehydration can be accelerated at _____
37 degrees Celsius
Excellent substitute for ethanol, with less shrinkage and hardening
Isopropanol
Identify this set-up with these given materials:
Forceps Tissue basket
Specimen label Pencil
Tissue capsule 80% alcohol
95% alcohol 100% alcohol
Chloroform Melted paraplast
Dehydration, Clearing and Infiltration
What is the specimen used?
Formalin-fixed tissue
After dehydration and clearing, how many hours do we impregnate the melted paraffin?
2 hours
Dehydration is best accomplished with the use of series of progressively increasing concentration beginning with ____
60%
What is the typical dehydration sequences for specimens not more than 4mm thick?
70% ethanol in 15 min
90% ethanol in 15 min
100% ethanol in 15 min
100% ethanol in 15 min
100% ethanol in 30 min
100% ethanol in 45 min
What is the typical clearing sequence for specimens not more than 4mm thick?
Xylene in 20 min
Xylene in 20 min
Xylene in 45 min
What is the typical paraffin infiltration sequence of paraffin for specimens not more 4mm think?
Paraffin wax in 30 min
Paraffin wax in 30 min
Paraffin wax in 45 min
The volume of the dehydrating agents should be at least ____ the volume of the tissue
10 times
Miscible with alcohol, xylene, and paraffin reducing the need for separate clearing steps
Dioxane
Universal solvent that dehydrates and clears tissue simultaneously
Tetrahydrofuran
What temperature speeds up dehydration used for urgent biopsies
37 degrees Celsius
Fill out the boiling points for each:
A. Butanol
B. Tertiary Butanol
C. Acetone
D. Cellosolve
E. Triethylphosphate
A. 117.7 degrees Celsius
B. 82.8 degrees Celsius
C. 56 degrees Celsius
D. 156.4 degrees Celsius
E. 215 degrees Celsius
