EMBEDDING

Question 1

Fill in the blanks to consider these proper tissue orientation

Elongated tissue = _______
Fragments = ________
________ = Embedded on end
________ = Right angle to the surface
Hard tissue = ______

Answer 1

Across the block
Clustered toward the center of the block
Tissue with lumen
Tissue with an epithelial surface
Embedded diagonally

Question 2

Specimen used in embedded and trimming

Answer 2

Well-infiltrated tissue

Question 3

Fill in the correct abbreviations of each specimen

Liver
Kidney
Lung
Stomach
Pancreas
Skin
Heart
Bone Marrow

Answer 3

LIV
KID
LNG
STM
PANC
SKN
HRT
BM

Question 4

Provides superior results for light microscopy, especially for hard tissues like bone and thin sections

Answer 4

Plastic (resin)

Question 5

Used as embedding medium for delicate specimens and frozen tissue sections

Answer 5

Gelatin

Question 6

Criteria for properly cooled paraffin

The crystals of paraffin are _____ and _____ with each other

The paraffin will appear _____ and ____

There is no _____ of paraffin

Answer 6

Small; contiguous
Clear; homogeneous
Layering

Question 7

How are tissues properly arranged? (3 things to consider)

Answer 7

In precise position in the mold during embedding
On the microtome before cutting
On the slide before staining

Question 8

This impregnation and embedding medium is not recommended for fatty tissues

Answer 8

Paraffin wax