Things I Forget Flashcards

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1
Q

What concentration of rbc suspension do we use for our Abo grouping

A

3-5% suspension rbcs in saline

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2
Q

How much of your reagent red cells do you add to gels?

A

50 ul reagent red cells

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3
Q

How much plasma do you add to gels?

A

25ul

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4
Q

What suspension are your rbc reagents for the antibody screen?

A

0.8%

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5
Q

In your own words how do you carry out an antibody screen using gels

A

Get your green AHG gel

Get your pipette and pipette tips

Get your reagent rbcs, add 50ul of 0.8% suspension to the wells

Get your patient plasma/serum and add 25ul to the wells

Incubate at 37 degrees for 15 mins and centrifuge

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6
Q

Define a forward group

A

Using known antiserum (eg. Anti-A, Anti-B, Anti-A,B)
to test for the presence of antigens on patient cells

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7
Q

Define a reverse group

A

Using reagent red cells of a particular ABO type
(eg A cells and B cells) to confirm the presence of
appropriate ABO antibodies in a patients
Plasma/Serum

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8
Q

What saline suspension of rbcs do you use for all tubes

A

3-5%

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9
Q

What saline suspension of rbcs do you use when adding rbcs for the reverse group in gels

A

0.8 to 1%

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10
Q

When carrying out your Rh phenotyping for patients in tube, what controls do you use?

A

You select two cells from the BioRad Screening cells as appropriate

You want a range of results between the two controls to cover your tests

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11
Q

What are your controls for the 3 panel antibody screen?

A

A weak anti D
An inert AB serum

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12
Q

What do you need to remember for the 3 panel antibody screen?
(4)

A

You need x2 LISS/Coombs Gels (Green lines on gel) -> one for your patient and one for your controls

You need 3 BioRad reagent cells (I, II, III)

Controls = 25ul weak anti-D or 25ul Inert AB to each reagent cell (50ul) on control gel i.e. no patient

Needs to be incubated for 15-20 mins at 37 degrees

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13
Q

What do you need to remember for the ID of unexpected antibodies gels?

A

You need x2 LISS/COOmbs gel cards (green)

You need x2 Enzyme Panel - NaCl Gels (red)

You need your BioRad Antibody ID panel - untreated (white cap)

You need you BioRad antibody ID panel - papanised (red cap)

Need to add 50ul of each rbc and 25ul patient plasma to each

+ Control needed for untreated and treated panel -> 25ul weak anti-D with 50ul RhD positive cell (pick from panel)

Need to incubate at 37 degrees for 15 minute

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14
Q

What is the shear plane?

A

The positive cloud surrounding the negative charge of rbcs

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15
Q

What is the zeta potential?

A

Potential = difference between two charges
Zeta potential = electrical potential of the slipping plane

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16
Q

Why do you need to add AHG for antibody screens?

A

IgM is able to fill the gap between rbcs and cause agglutination

IgG is unable to do these - too small

17
Q

What antigens need homozygous expression to be excluded in an antibody screen

A

MNS
Duffy
Kidd

18
Q

Papain destroys which antigens

A

MNS
DUFFY